Unit 4 - Genetic information, variation and relationships between organisms
Resumen
Summary PCR and cloning notes
16 vistas 0 veces vendidas
Grado
Unit 4 - Genetic information, variation and relationships between organisms
Institución
AQA
These notes helped me get into medical school, I completed my a levels at Guildford high school which consistently ranks among the top schools in the UK and is known for their academic rigour. I made these notes as a summary of the power points that we were taught off of.
Unit 4 - Genetic information, variation and relationships between organisms
Todos documentos para esta materia (131)
Vendedor
Seguir
anastazjaohearn
Vista previa del contenido
resistance to
> MRNA is easier to find desired gene. reverse gene cloning ↓
A
& J
asmid I
plrecognition
desire set
g
no introns transcriptase makes (copies) CDNA
. Restriction Invitro (PCR) in vivo
·
endonucleases cut up CDNA
making sticky ends
. separate DNA95% plasmids (vector)
g
resistance
fragments to plasmids
. removed with To test transfer,
DNA
ligase joins up annealing primers gene a to B
%
55 C restriction will be resistant to
B
(sticking) enzyme
gene machine shows base sequence based ·
tag polymerase ·
join Vector and DNA and not to A
*
<
g
on a protein PCR
C an
. be used to amplify adds nucleotides 72%
fragment with DNA ligase
Interrupted
↑ >
-
fast ·
Recombinant DNA introduced
to bacterium
Selectable
markers
genetic
a
universal
gene therapy
>
-
non-overlaping
& degenerate
Tag
polymerase
↓
will not
denature
at 95%
-
genetic fingerprinting
extraction from sample
digestion < restriction endonucleases
separating -
gel electrophoresis
*
Annealing
separation n transfered to nylon membrane
risk of contaminated DNA
amplifying a hybridisation< radioactive probes attach
·
development placed onto
xray film
Los beneficios de comprar resúmenes en Stuvia estan en línea:
Garantiza la calidad de los comentarios
Compradores de Stuvia evaluaron más de 700.000 resúmenes. Así estas seguro que compras los mejores documentos!
Compra fácil y rápido
Puedes pagar rápidamente y en una vez con iDeal, tarjeta de crédito o con tu crédito de Stuvia. Sin tener que hacerte miembro.
Enfócate en lo más importante
Tus compañeros escriben los resúmenes. Por eso tienes la seguridad que tienes un resumen actual y confiable.
Así llegas a la conclusión rapidamente!
Preguntas frecuentes
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
100% de satisfacción garantizada: ¿Cómo funciona?
Nuestra garantía de satisfacción le asegura que siempre encontrará un documento de estudio a tu medida. Tu rellenas un formulario y nuestro equipo de atención al cliente se encarga del resto.
Who am I buying this summary from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller anastazjaohearn. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy this summary for 3,70 €. You're not tied to anything after your purchase.
