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Pathogenic Microbiology Test 4 Questions And Verified Correct Answers

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  • BOC Microbiology

What are some characteristics of the Enterobacteriaceae? - Answer all members are gram negative bacilli or coccobacilli all members are cytochrome oxidase negative all members grow on MacConkey agar Do all enterobacteriaceace members ferment lactose? - Answer No Swarming colonies on...

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Pathogenic Microbiology Test 4
Questions And Verified Correct Answers.
What are some characteristics of the Enterobacteriaceae? - Answer all members are gram negative
bacilli or coccobacilli

all members are cytochrome oxidase negative

all members grow on MacConkey agar



Do all enterobacteriaceace members ferment lactose? - Answer No



Swarming colonies on sheep blood agar would lead one to suspect: - Answer Proteus



What organisms produce H2S or gas? - Answer Salmonella

Proteus

Citrobacter



Does Shigella produce H2S or gas? - Answer No



The production of deaminase is found in which of the following groups of bacteria? - Answer Proteus,
Providencia, and Morganella



The most frequently implicated species of Shigella in the United States is: - Answer S. sonnei



Enterohemorrhagic E. coli (EHEC) is: - Answer Also known as verocytoxic E. coli

May be associated with hemolytic uremic syndrome

May often serotype as 0157:H7



Is the H antigen found in the cell wall? - Answer No

,On Hektoen Enteric agar, Shigella appears as: - Answer Clear or colorless colonies.



Are members of the tripe Klebsielleae VP positive or negative? - Answer Positive



Is Proteus mirabilis indole positive or negative? - Answer Negative



In the ____________ __________ pathway for glucose fermentation, acetoin is detected. - Answer
Voges Proskauer



Rapid urease activity is characteristic of the genus _________. - Answer Proteus



___________ ___________ causes plague, which is transmitted through fleas and rodents. - Answer
Yersinia pestis



__________ ____________ produces DNAse, lipase, gelatinase and sometimes a red pigment. - Answer
Serratia marcescens



________ media is used to isolate Yersinia enterocolitica. - Answer CIN

Cefsulodin-irgasin-novobiocin



A surgical wound specimen produces pink colonies on MacConkey agar. There is no growth on CNA and
abundant flat, spreading gray mucoid colonies on blood agar.



The next four questions are talking about this case study above.



This specimen contains: - Answer A gram negative bacillus that ferments lactose



The TSI is yellow over yellow with cracks but no blackening. This would be interpreted as: - Answer
Ferments glucose with gas production, ferments lactose and/ or sucrose

, The IMVC reactions are - - + + which would eliminate __________ as a possible organism. - Answer E.
coli



The organism is nonmotile, LDC +, ODC -, ADH - which would most likely indicate that the isolate is: -
Answer Klebsiella pneumoniae



129) Infection of the urinary tract is most frequently associated with:

a. Staphylococcus aureus

b. Escherichia coli

c. Enterococcus faecalis

d. Serratia marcescens - Answer b (>80% of uncomplicated UTIs are caused by E coli.)



130) MacConkey media for screening suspected cases of hemorrhagic E coli O157:H7 must contain:

a. indole

b. citrate

c. sorbitol

d. lactose - Answer c (Sorbitol replaces lactose in MacConkey. E coli 0157:H7 does not ferment sorbitol,
whereas other species of E coli are positive for fermentation of sorbitol. This makes the media a good
screen for 0157:H7.)



131) Members of the family Enterobacteriaceae share which one of the following characteristics?

a. produce cytochrome oxidase

b. ferment lactose

c. produce beta-hemolysis

d. reduce nitrate to nitrite - Answer d (Members of Enterobacteriaceae are oxidase negative, ferment
glucose, and reduce nitrate to nitrite.)



132) Which one of the following genera is among the least biochemically reactive members of the
Enterobacteriaceae?

a. Proteus

b. Pseudomonas

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