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NWHSU Biochem 1 - Exam 2 (Enzymes and Carbohydrates) Questions and Answers
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NWHSU Biochem 1 - Exam 2 (Enzymes and Carbohydrates)
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NWHSU Biochem 1 - Exam 2 (Enzymesand Carbohydrates)
Enzymes ii- iiAnswers ii--Increase iireaction iirate ii(Vo) ii- ii"velocity"; iiM/min iior iimol/min
-Are iinot iiused iiup iiin iireaction
Enzyme iifunctions iiunder iimild iiconditions ii- iiAnswers ii-- iitemperature
- iipH
- iipressure
Structure iiof iiEnzymes ii- iiAnswers ii-- iivast iimajority iiare iiproteins
- iimany iineed iia iinon-amino iiacid iicomponent iito iibe iifunctional
Function iiof iiEnzymes ii- iiAnswers ii-Enzymes iiincrease iivelocity iiby iiwhich iiproduct iiis
iiformed iiover iitime
Enzyme iikinetics ii- iiAnswers ii-- iiHelps iiunderstand iiand iipredict iienzymatic iireactions
iiand iifactors iiaffecting iithem
- iiStudying iireaction iirate iivs ii[S] iirelationships
ADH ii- iiAnswers ii-Alcohol iiDehydrogenase
Reaction iicatalyzed iiby iiADH ii- iiAnswers ii-Ethanol ii(substrate) ii- ii(remove ii2 iiC-) ii->
iiAcetaldehyde ii(Product)
Hyperbola ii- iiAnswers ii-What iiis iithe iiADH ii(Vo iivs ii[S]) iiplot iiform?
at iithe iiactive iisite iiinstead iiof iithe iisubstrate ii- iiAnswers ii-A iicompetitive iiinhibitor
iibinds...
reversible iinon-covalent ii- iiAnswers ii-Feedback iiinhibition iiis iian iiexample iiof ii______
iiinhibition
vitamin iiK iidependent ii- iiAnswers ii-Glutamate iicarboxylase iiinvolved iiin iiblood iiclotting
iiis ii_____
Noncovalent, iireversible, iienzyme iiactivation ii- iiAnswers ii-allosteric
Proteases iiin iithe iismall iiintestine iiget iiactivated iiby... ii- iiAnswers ii-proteolytic iicleavage
iiof iiC- iiand/or iiN- iiterminus
, 3 iitypes iiof iireversible iiinhibition ii- iiAnswers ii-competitive
uncompetitive
mixed
Competitive iiInhibition ii- iiAnswers ii-Inhibitor iihas iito iihave iisimilar iistructure iito
iiSubstrate ii(e.g. iimethanol ii(CH3OH) iiis iiseminal iito iiethanol ii(CH3CH2OH)
When iithe iilines ii(with iior iiwithout) iiintersect iion iiy-axis iion iithe iiLB iigraph, iiwhich
iiinhibition iiis iiit? ii- iiAnswers ii-competitive
Describe iiwhat iiyou iiwould iisee iiin iia iiMM-plot iiof iicompetitive iiinhibition. ii- iiAnswers ii-
The iiVmax iiof iiboth ii(with iior iiwithout) iiwill iibe iithe iisame iiand iithat iithe iiKm iiwill iiincrease
iionce iithe iiinhibitor iiis iiattached.
Uncompetitive iiInhibition ii- iiAnswers ii-- iiInhibitor iihas iiit's iiown iibinding iisite ii(it's iinot iithe
iiactive iisite)
- iiInhibitor iican iionly iibind iito iithe iiEnzyme iiSubstrate ii(ES) iicomplex iinot iiEnzyme iialone
On iia iiLB-graoh, iiwhich iiinhibition iiis iishown iiwhen iiboth iilines iiruns iiparallel iito iianother
iiand iinever iiintersects? ii- iiAnswers ii-Uncompetitive iiInhibition
Describe iiwhat iiis iiseen iiin iian iiuncompetitive iiMM-plot iigraph? ii- iiAnswers ii-The iiVmax
iiand iiKm iiboth iidecrease iifrom iiES iiwithout iiInhibitor iito iiES iiwith iiInhibitor
Mixed iiInhibition ii- iiAnswers ii-- iiInhibitor iihas iiit's iiown iibinding iisite ii
- iiCan iibind iito iieither iiES iior iiE
On iia iiLB iigraph, iiwhich iiinhibition iiis iishown iiwhen iilines iiintersect iiin ii2nd iiquadrant? ii-
iiAnswers ii-Mixed iiinhibition
Describe iiwhat iiis iiseen iiin iia iimixed iiMM-plot iigraph. ii- iiAnswers ii-The iiVmax
iidecreases iiand iithe iiKm iiincreases iionce iithe iiinhibitor iiattaches
Enzymes iiaffects... ii- iiAnswers ii-rate iiof iireaction
What iihappens iito iiactivation iienergy iiin iipresence iiof iian iienzyme? ii- iiAnswers ii-it
iidecreases
What iiis iiKm? ii- iiAnswers ii-Michaelis-menton iiconstant iithe ii[S] iiwhere iithe iirate iiis ii1/2
iiVmax
What iiis iithe iiInverted iiMM iiequation? ii- iiAnswers ii-(1/Vo) ii= ii(Km ii+ ii[S]) ii/ ii(Vmax ii. ii[S])
What iiare ii2 iitypes iiof iinon iicovalent ii(reversible) iiregulation? ii- iiAnswers ii-MM iitype
iiinhibitors ii(competitive, iiuncompetitive, iimixed)
Allosteric iiregulation ii(removed iifrom iiactive iisite, iichange iiof iiconcentration iiof iienzyme)
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