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EMSA TEST QUESTIONS AND ANSWERS
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full form of EMSA - ANSWER ELECTROPHORETIC MOBILITY SHIFTASSAY
What is EMSA? - ANSWER It's a technique used to study the interaction
between DNA & protein. It's a simple, efficient & sensitive technique.
Principle of EMSA - ANSWER 1. DNA moves through the gel faster when not
bound with protein.
2. Reduction in electrophoretic mobility shows that a complex is formed
between DNA & PROTEIN.
3. Can be used to identify DNA binding protein present in nuclear cell extract.
example: transcription factor.
what is the transcription factor? - ANSWER it's a molecule involved in
regulating gene expression. they are usually proteins also they can consist of
short, non-coding RNA.
transcription factors are the only ones in the means by which our cells express
different combinations of genes. allowing for differentiation into various types
of cell, tissue, organ that make our bodies.
5 basic steps in EMSA protocol? - ANSWER 1. Preparation of purified or crude
protein sample
2.preparation of nucleic acid
3.binding reactions
4.
where are transcription factor made? - ANSWER
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