100% de satisfacción garantizada Inmediatamente disponible después del pago Tanto en línea como en PDF No estas atado a nada
logo-home
Summary Cell fractionation and ultracentrifugation Year 1 A level Biology AQA 3,70 €   Añadir al carrito

Resumen

Summary Cell fractionation and ultracentrifugation Year 1 A level Biology AQA

 5 vistas  0 veces vendidas
  • Grado
  • Institución

Year 1 Biology AQA A level notes. Achieved an A* in my final exam using these notes. They are based on the mark scheme requirements.

Vista previa 1 fuera de 1  páginas

  • 2 de noviembre de 2024
  • 1
  • 2024/2025
  • Resumen
avatar-seller
Cell fractionation and ultracentrifugation
summary notes
22 September 2022 14:20

Cell fractionation


- Cells are broken up and the different organelles they contain are
separated out
- Tissue is placed in cold, buffered solution of the same water
potential as the issue

Solution must be
1. Cold to reduce enzyme activity so organelles are not broken down
2. Same water potential as the tissue so that organelles do not burst
or shrink due to osmosis (isotonic)
3. Buffered so that pH does not change as this could change
structure of organelles or affect function of enzymes (ie denature
them)



Stage 1- Homogenation
- Cells broken up by homogeniser which is like a blender- breaks the
plasma membrane
- Releases organelles from cell
- Homogenate is resultant fluid that is filtered through a gauze to
remove any complete cells or debris

Stage 2- Ultracentrifugation
- Fragments in filtered homogenate are separated into centrifuge
machine
- Spins them at high speed to create centrifugal force
Process of ultracentrifugation:
- Tube of homogenate place in centrifuge at slow speed
- Heaviest organelles, the nucleus, forced to bottom of tube- form
sediment
- Fluid at the top, supernatant, is removed, leaving sediment
- Supernatant is transferred to another tube and spun in centrifuge
at faster speed than before (again so the heaviest organelles
forced to the bottom of the tube)




Mass

Los beneficios de comprar resúmenes en Stuvia estan en línea:

Garantiza la calidad de los comentarios

Garantiza la calidad de los comentarios

Compradores de Stuvia evaluaron más de 700.000 resúmenes. Así estas seguro que compras los mejores documentos!

Compra fácil y rápido

Compra fácil y rápido

Puedes pagar rápidamente y en una vez con iDeal, tarjeta de crédito o con tu crédito de Stuvia. Sin tener que hacerte miembro.

Enfócate en lo más importante

Enfócate en lo más importante

Tus compañeros escriben los resúmenes. Por eso tienes la seguridad que tienes un resumen actual y confiable. Así llegas a la conclusión rapidamente!

Preguntas frecuentes

What do I get when I buy this document?

You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.

100% de satisfacción garantizada: ¿Cómo funciona?

Nuestra garantía de satisfacción le asegura que siempre encontrará un documento de estudio a tu medida. Tu rellenas un formulario y nuestro equipo de atención al cliente se encarga del resto.

Who am I buying this summary from?

Stuvia is a marketplace, so you are not buying this document from us, but from seller arabellasherratt. Stuvia facilitates payment to the seller.

Will I be stuck with a subscription?

No, you only buy this summary for 3,70 €. You're not tied to anything after your purchase.

Can Stuvia be trusted?

4.6 stars on Google & Trustpilot (+1000 reviews)

45,681 summaries were sold in the last 30 days

Founded in 2010, the go-to place to buy summaries for 14 years now

Empieza a vender
3,70 €
  • (0)
  Añadir