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Summary Calibrating microscopes
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Year 1 Biology AQA A level notes. Achieved an A* in my final exam using these notes. They are based on the mark scheme requirements.
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Microscopes summary notes29 September 2022 12:52
Graticule
- Graticule is small disc that has an engraved ruler-
on the eyepiece of the microscope
- Does not move when you move specimen
- Size does not change when you change the
objective lens
- One division of the eyepiece represents a
different length depending on magnification
Stage Micrometre
- Slide with a scale marked on it
- Placed on stage of a microscope
- Usually 1mm long and marked in hundredths
- Used to find out how many micrometres each
graticule unit represents
Calibrating:
- Specimen removed from stage and replaced with
stage micrometre
- Use scales together to work our each graticule
unit
- Graticule then used as a ruler in the field of view
Method:
- Place a stage micrometer on the stage of the
microscope.
- Line up one of the divisions on the eyepiece
graticule with a fixed point on the stage
micrometer.
- Count the number of divisions on the eyepiece
graticule that correspond with a set
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