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LSU CHEM 317 BIOCHEMISTRY I FINAL EXAM Questions and Answers Louisiana University
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LSU CHEM 317 BIOCHEMISTRY IFINAL EXAM Questions and Answers
Louisiana University
CHEM317-001-202120 Biochemistry I
Started on Monday, 23 November 2020, 8:00 AM
State Finished
Completed on Monday, 23 November 2020, 10:30 AM
Time taken 2 hours 29 mins
Marks 43.00/67.00
Grade 129.00 out of 201.00 (64%)
Question 1
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The antibiotic fluconazole is used against pathogenic fungi. After long-term treatment,
mutations of the organisms can arise. One yeast strain was mutated. The kinetics of the
enzyme that is the target of fluconazole, lanosterol 14-demethylase (14-DM), were
determined in the wild type and the mutant yeast, and curves shown in Figure 1a and
1b were obtained. In order to determine the mutations in 14-DM, the amino acid
sequences of 14-DM from the wild type and the mutant strain were determined. Figure
2 shows a comparison of the part of the sequences where differences were found.
,Which of the following is the effect of fluconazole on the lanosterol 14-DM? Which of the
following amino acid changes shown in Figure 2 is most likely to alter sensitivity to
fluconazole?
Select one:
a. Noncompetitive; Lys → Arg
b. Competitive inhibition; Lys → Arg
c. Noncompetitive; His → Thr
,d. Competitive inhibition; His → Thr
e. Either competitive inhibition or noncompetitive inhibition; Ether Lys → Arg or His → Thr
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The correct answer is: Noncompetitive; His → Thr
Question 2
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Protein concentration can be quantitated by visible spectrometry. In one experiment,
the standard curve below was produced. A 10 μL aliquot of protein isolate was diluted
to a final volume of 100 μL with buffer. The absorbance of this dilution was 0.61. What
is the protein concentration of the original isolate?
Select one:
a. 0.151 mg·mL-1
b. 1.51 mg·mL-1
c. 0.0151 mg·mL-1
d. 15.1 mg·mL-1
e. 151 mg·mL-1
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Your answer is incorrect.
The correct answer is: 0.151 mg·mL-1
Question 3
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Millions of children that depend primarily on rice as a food staple become blind each
year due to vitamin A deficiency. Humans cannot synthesize vitamin A and must have a
supply of vitamin A or vitamin A precursor like beta-carotene in their diet. You want to
create a strain of yeast that has the complete beta-carotene pathway so you can easily
study the biochemistry of beta-carotene and vitamin A synthesis. You have a yeast
strain that has 6 of the required 7 enzymes for beta-carotene synthesis. You need to
provide these yeast cells with the missing enzyme psy2 from daffodils. You are also
given some DNA sequence from the psy2 locus, cloning vector 2 and restriction
enzymes as shown below.
To complete production of the yeast strain capable of synthesizing beta-carotene, you
decide to use PCR to amplify the psy2 coding sequence based on the flanking sequence
shown below
Select one:
a. Sense primer: 5’-AGGCCG-3’; Anti-sense primer: 5’-GCCGGA-3’
b. Sense primer: 5’-AGGCCG-3’; Anti-sense primer: 5’-ACCGGG-3’
c. Sense primer: 5’-TCCGGC-3’; Anti-sense primer: 5’-ACCGGG-3’
d. Sense primer: 5’-TCCGGC-3’; Anti-sense primer: 5’-GCCGGA-3’
e. Sense primer: 5’-TCCGGC-3’; Anti-sense primer: 5’-TCCGGC-3’
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Your answer is correct.
The correct answer is: Sense primer: 5’-TCCGGC-3’; Anti-sense primer: 5’-GCCGGA-3’
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