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Microbiology Lab UTA Final Exam | Questions And Answers Latest {} A+ Graded | 100% Verified
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Microbiology Lab UTA Final Exam | Questions And Answers Latest {2024- 2025} A+Graded | 100% Verified
How is Media made? - 5 g. T.S.A. and 125 mL Di water, cover flask with foil, affix autoclave tape,
autoclave, wait 10-20 min to cool, pour into petri-dish to thickness of lid. or stop when covers bottom.
Agar - linear polysaccharide polymer derived from agarose in red algae.
What does agar do? - increases solidity, exhibits hysteresis- gels and melts at different temps. Melts
above 85-90 and solidifies at 40-45.
What is liquid media? - commonly called broths, doesn't contain agar, easy to use, allows observation of
growth patterns.
Advantages to using semi-solid media - To test oxygen requirements, to test motility.
Fastidious - only grows in a fortified culture media under specific conditions. The more _____________
the organism, the more specific ingredients a medium must have. Uses defined media.
Why use solid media? - The maintenance and growth of stock cultures, to isolate single colonies, to
ensure purity.
T.S.A. Plate - Tryptic Soy Agar most common medium used in lab. Undefined media that contains casein
digest, soybean digest, NaCl, and agar.
What are the 4 biochemical families all living things are composed of? - Proteins, Carbohydrates, lipids,
nucleic acids
How much water does a bacteria cell consist of? - 70%
Autoclave - sterilizes with steam at 121* C, pressure at 15 PSI for at least 15 min. Tested weekly to daily.
,Reservoir - potential source of infection.
Normal commensal symbionts - S. pneumoniae and s. aureus
Environmentally acquired microbes - p. aeruginosa and b. cepacia
Magnifications of our microscopes - scanning: 4x-40, low: 10x-100, high: 40x-400, oil immersion: 100x-
1000
What is resolving power? - How close two objects can be and still be distinguished as separate.
What is the optimum resolution of the microscope using oil immersion? - .2 micrometer.
How much does each microscope cost? - $1600
convex - Morphology of a domed colony
raised - flatter dome than convex
flat elevation -
crateriform - hole in convex colony
Umbonate - Morphology of a colony with a raised center
Rhizoid - Morphology of a branching colony
lobate - margin with lobes
, undilated margins - irregular but not lobed
curled - wave pattern on margin
entire margin - even smooth all around
Filiform Growth - Agar slant. Colony exhibits a dense/opaque surface with a defined edge.
Friable Growth - Agar slants. Colony exhibits crusty, irregular edges.
Spreading edges - motile organism
Human error in inoculation - What is responsible for most irregularities in agar slants?
Five I's - inoculation, incubation, isolation, inspection, identification
Flocculence - Clumpy colonial aggregates seen in broth media. streptococcus
Pellicle - some colonies float on top of broth. Mycobacterium phlei
Spirillum - Bacteria shaped like a coil.
Spirochete - Bacteria shaped like a long, wavy strand of hair.
Vibrio - Bacteria shaped like a comma
Pleomorphic - Bacteria with an irregular or morphing shape (star-shaped, square, etc).
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