MOLECULAIRE BIOLOILOGIE:
ALU-INSERTIE IN TPA-GEN
Biotechnologie BLC 3
, uitvoering labo: 26/11/2021
Inhoud
1. Summary............................................................................................................................................2
1.1 Goal..............................................................................................................................................2
1.2 Principle.......................................................................................................................................2
1.3 Method........................................................................................................................................2
1.3.1 Extraction of DNA..................................................................................................................2
1.3.2 PCR.........................................................................................................................................2
1.3.3 Gel electrophoresis................................................................................................................2
2. DNA-extractie.....................................................................................................................................3
2.1 Voorbereiding en benodigdheden................................................................................................3
2.2 Werkwijze....................................................................................................................................3
3. Ploymerase chain reaction (PCR)........................................................................................................4
3.1 Voorbereidingen en benodigdheden...........................................................................................4
3.2 Werkwijze....................................................................................................................................5
4. Agarosegelelektroforese.....................................................................................................................6
4.1 Voorbereidingen en benodigdheden............................................................................................6
4.2 Werkwijze....................................................................................................................................7
5. Resultaten...........................................................................................................................................8
6. Conclusie............................................................................................................................................9
1
ALU-INSERTIE IN TPA-GEN
Biotechnologie BLC 3
, uitvoering labo: 26/11/2021
Inhoud
1. Summary............................................................................................................................................2
1.1 Goal..............................................................................................................................................2
1.2 Principle.......................................................................................................................................2
1.3 Method........................................................................................................................................2
1.3.1 Extraction of DNA..................................................................................................................2
1.3.2 PCR.........................................................................................................................................2
1.3.3 Gel electrophoresis................................................................................................................2
2. DNA-extractie.....................................................................................................................................3
2.1 Voorbereiding en benodigdheden................................................................................................3
2.2 Werkwijze....................................................................................................................................3
3. Ploymerase chain reaction (PCR)........................................................................................................4
3.1 Voorbereidingen en benodigdheden...........................................................................................4
3.2 Werkwijze....................................................................................................................................5
4. Agarosegelelektroforese.....................................................................................................................6
4.1 Voorbereidingen en benodigdheden............................................................................................6
4.2 Werkwijze....................................................................................................................................7
5. Resultaten...........................................................................................................................................8
6. Conclusie............................................................................................................................................9
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