TAMU bio 111 lab exam 2 review 2024/2025 Questions And Answers 100% Solved | 100% Pass

TAMU bio 111 lab exam 2 review 2024/2025 Questions And Answers 100% Solved | 100% Pass What is the difference between the coding and non-coding region? - Coding region: comprised of genes that encode proteins (instructions that determine traits) and makes up 1-2% of human DNA. • Noncoding region: makes up 98-99% of human DNA, contains STRs and is responsible for regulatory functions such as gene transcription. What are STRs? - Short-tandem repeats (STRs) are unique repeating patterns of the same nucleotide sequence. They can be used to differentiate one person from another. What is a DNA profile? - A DNA profile is a specific pattern of DNA attributes that is obtained in the lab and can be used to identify an individual. • DNA profiling is the process of determining an individual's DNA characteristics (profile) from a sample of bodily tissue What does PCR stand for? What is the purpose of PCR? - Polymerase Chain Reaction • Purpose is to amplify DNA. In forensics, this can be used to obtain more DNA from a sample that would otherwise be too small to analyze. What is inside the master mix? - 3 pairs of primers, loading dye, nucleotides (dNTPs), Taq polymerase, mix buffer, and water. DNA sample is added to master mix.