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Virology exam 2 Questions with Correct Answer2
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Virology exam 2 Questions with Correct Answer2
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Virology exam 2 Questions with Correct AnswersWhich of the following process form the 5` mRNA cap structure in the
Influenza Virus? Correct Answer-The 5`cap is cleaved and snatched by
the virus from host nuclear pre-mRNAs
What strategy is used by the Poliovirus to make many proteins from a
single mRNA? Correct Answer-proteolytic cleavage of the poly protein
Which of the following group of viruses usually do NOT pack RNA
dependent RNA polymerase in the virus particle? Correct Answer-plus
strand RNA viruses
Coronavirus and Retrovirus are two unique viruses among the plus-
strand RNA viruses. Which of the following feature make them unique?
Correct Answer-They are the only plus-strand RNA viruses with
encapsidated genomic RNA in the form of the nucleocapsid
Which of the following is NOT true about viral mRNAs? Correct
Answer-Uncapped viral mRNAs can't be translated into protein by the
ribosomes
Actinomycin D was utilized in the experiments that lead to the discovery
of the viral RNA polymerase. What was the principle of the experiment?
Correct Answer-In the presence of NTP and Actinomycin D; which
inhibited cellular DNA dependent RNA polymerase; RNA synthesis
occurred in the virally infected cell extract.
,The mRNAs are synthesized by the Reovirus in which of the following
structural state of the virus? Correct Answer-When the core particle is
released into the cytoplasm
Which of the following process acts as the primary "switch" from
Vesicular Stomatitis Virus (VSV) mRNA synthesis to genome RNA
synthesis? Correct Answer-Encapsidation of the "leader RNA" by N
protein and association of N protein with RNA polymerase which reads
through the intergenic region
The catalytic site of the RNA dependent RNA polymerase is present in
which of the following domain? Correct Answer-the palm
Which of the following type of metal ion coordinate the incoming
nucleotide in a polymerization reaction catalyzed by polynucleotide
polymerase class of enzymes like RNA or DNA polymerases? Correct
Answer-di-valent metal ion
Which of the following acts as a primer for the initiation of poliovirus
mRNA synthesis? Correct Answer-the VPg protein and covalently
bonded two uridine residues
Which of the following group of viruses does NOT have naked RNA in
the virus particle? Correct Answer-minus strand RNA viruses
Which of the following is the major difference between the Vesicular
Stomatitis Virus (VSV) and Influenza Virus mRNA synthesis? Correct
, Answer-VSV mRNAs are synthesized in the cytoplasm whereas the
Influenza virus mRNAs are synthesized in the nucleus.
Which RNA polymerase mode of action "model" explains the VSV
mRNA synthesis and attenuation at intergenic regions? Correct Answer-
Stop-start model
Which of the following process describes the polyadenylation of
mRNAs in Vesicular Stomatitis Virus (VSV)? Correct Answer-Slippage
of the RNA polymerase and recopying over a seven residue uridine (U7)
at each intergenic region
Which of the following attributes to the specificity of RNA polymerases
towards NTPs over dNTPs? Correct Answer-The 2` hydroxyl of the
ribose sugar
How are the Influenza virus and VSV RNA synthesis similar? Correct
Answer-1. The switch from mRNA to genome RNA synthesis is
controlled by an RNA-binding protein
2. Polyadenylation occurs at a short stretch of U residues
3. Viral mRNAs are shorter than (−) genome RNA
Which of the following group of viruses usually pack RNA dependent
RNA polymerase in the virus particle? Correct Answer-double stranded
RNA viruses and minus strand RNA viruses
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