BMES 5024 Midterm UPDATED ACTUAL Questions and CORRECT Answers
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BMES 5024
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BMES 5024
BMES 5024 Midterm UPDATED ACTUAL
Questions and CORRECT Answers
Andreas Vesalius () - CORRECT ANSWER- First most accurate and
comprehensive anatomical text "De Humani Corporis Fabrica"
Seven Volumes on the structure of the human body
William Harvey () - CORRECT ANSWER- "On the Motion of the H...
BMES 5024 Midterm UPDATED ACTUAL
Questions and CORRECT Answers
Andreas Vesalius (1514-1564) - CORRECT ANSWER- ✔✔First most accurate and
comprehensive anatomical text "De Humani Corporis Fabrica"
Seven Volumes on the structure of the human body
William Harvey (1578-1657) - CORRECT ANSWER- ✔✔"On the Motion of the Heart and
Blood in Animals"
The first accurate theory of how the heart circulatory system operate.
Antonie van Leeuwenhoek (1632-1723) - CORRECT ANSWER- ✔✔-Discovered bacteria,
free-living and parasitic microscopic protists, sperm cells, blood cells, microscopic
nematodes....
-Opened up entire world of microscopic life
Edward Jenner (1749-1823) - CORRECT ANSWER- ✔✔Developed the first effective
vaccine against smallpox
Crawford Williamson Long (1815-1878) - CORRECT ANSWER- ✔✔Pioneering physician
who is credited with discovering anesthesia. First surgical use of ether
William Rontgen (1845-1923) - CORRECT ANSWER- ✔✔Discovered X-rays
Received first Nobel Prize (1901)
Ross Granville Harrison (1870-1959) - CORRECT ANSWER- ✔✔Discovered a method of
tissue culture. Culture of frog nerve tissue in blood clot
Nikolay Anichkov (1885-1964) - CORRECT ANSWER- ✔✔Discovered the role of
cholesterol in coronary artery disease
Alexander Fleming (1881-1955) - CORRECT ANSWER- ✔✔Discovered Penicillin. Novel
prize in physiology and medicine (1945)
,Maurice Wilkins - CORRECT ANSWER- ✔✔Discovered the structure of DNA. Novel prize
in physiology and medicine (1962)
Model Systems - CORRECT ANSWER- ✔✔-Basic cell biology & biochemistry
-Interactions between disease-causing agents & cells
-Drug effects on cells, aging process, nutritional studies, etc.
Toxicity Testing - CORRECT ANSWER- ✔✔Studies the effects of new drugs, cosmetics,
and other chemicals on survival and growth in variety of cell types.
Virology - CORRECT ANSWER- ✔✔-Basic research on how viruses infect organisms
-clinical detection and isolation of viruses
Cell culture applications - CORRECT ANSWER- ✔✔-genetic engineering
-gene therapy
-drug screening & development
-cell based manufacturing
Primary cell cultures - CORRECT ANSWER- ✔✔primary neurons, HUVEC
-obtain directly from normal animal tissues
-limited life spam
-usually retain characteristics of in vivo cells in normal tissues
Continuous Cell Cultures - CORRECT ANSWER- ✔✔Transformed (immortalized) cell lines
BV-2, HMEC-1
-propagate indefinitely
-transformed using viral oncogenes or chemical treatments
-retain little of original in vivo characteristics
, Cancer Cell Lines - CORRECT ANSWER- ✔✔-continuous
-single cell type propagated in culture for limited number of divisions
-usually retain characteristics of in vivo cells in tumor tissues
-can grow longer than primary cell lines
Explant culture step - CORRECT ANSWER- ✔✔- small pieces of tissue are attached to glass
or plastic culture vessel and placed in culture medium
-after a few days, individual cells move from the tissue explants out onto the culture vessel
surface
- cells begin to divide and grow
Enzymatic Dissociation step - CORRECT ANSWER- ✔✔-addition of digesting enzymes
(such as trypsin or collagenase) to the tissue fragments to dissolve the cement holding the
cells together
-resultant suspension of single cells are then placed into culture vessels containing culture
medium and allowed to grow and divide.
Isolation of HUVEC - CORRECT ANSWER- ✔✔1) cut one end with a sterile surgical blade
2) look for the vein (widest vessel), place needle into vein, and place the free end of the cord
inside the clamp and screw it in
3) wash, digest with collagenase solution, incubate
4) collect HUVEC, centrifuge, and obtain cell pellet. Add growth media and suspend cell
pellet.
Stroke Model - CORRECT ANSWER- ✔✔middle cerebral artery occlusion (MCAO)
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