Resume
Summary Leerdoelen Moleculaire diagnostiek
- Cours
- Établissement
- Book
Uitwerkingen van de leerdoelen die gegeven zijn bij het vak moleculaire diagnostiek.
[Montrer plus]
Livre connecté
Titre de l’ouvrage:
Auteur(s):
- Édition:
- ISBN:
- Édition:
Plus de résumés pour
-
Test Bank for Molecular Diagnostics Fundamentals, Methods, and Clinical Applications, 3rd Edition, Lela Buckingham.
-
Test Bank for Molecular Diagnostics Fundamentals, Methods, & Clinical Applications, 1st Edition, Maribeth L. Flaws, Lela Buckingham.
-
Samenvatting Lesstof Moleculaire Diagnostiek 2
Vendeur
S'abonner
annabellewiegers
Aperçu du contenu
Learning objectives Molecular DiagnosticsStudy task 1
Chapter 1: DNA
1. Describe the structure of DNA and nucleotides
Deoxyribonucleic acid (DNA) is a macromolecule of carbon, nitrogen, oxygen, phosphorous and
hydrogen atoms. It is assembled in units of nucleotides that are composed of a phosphorylated
ribose sugar and a nitrogen base. There are four nitrogen bases called nucleosides (bound to an
unphosphorylated sugar): adenine (A, purine), cytosine (C, pyrimidine), guanine (G, purine) and
thymine (T, pyrimidine), where C binds G (three hydrogen bonds) and A binds T (two hydrogen
bonds). These nitrogen bases are attached to a deoxyribose sugar, which forms a polymer with the
deoxyribose sugars of other nucleotides through a phosphodiester bond. Linear assembly of the
nucleotides makes up one strand of DNA, two strands comprise the DNA double helix. This helical
structure of DNA results from the physicochemical demands of the linear array of nucleotides in the
strand, as well as the surrounding chemical micro-environment, can affect the nature of the DNA
helix.
2. Describe the process of DNA replication and the function of the enzymes involved
The two DNA strands that make up a double helix have an antiparallel orientation. The DNA is
synthesized in the 5’ to 3’ direction by DNA polymerase, an enzyme (reads in the 3’ to 5’ direction)
that uses a template to determine which nucleotides to add to the chain.
Firstly, the double helix must be unwound by helicase, so that both strands can serve as a template.
The single strands are then elongated by hydrogen bonding of the complementary incoming
nucleotide to the nitrogen base. While
DNA replication on the leading strand
(3’ to 5’) proceeds in a continuous
manner, lagging strand (5’ to 3’) is
replicated in a discontinuous manner
with the use of Okazaki fragments
(small DNA fragments) by jumping
ahead a short distance (~1000 bases)
and then copies backwards.
DNA cannot be synthesized the novo; a preceding base must be present to provide the hydroxyl
group (deoxyribose 3’-hydroxyl oxygen). This base is provided by another enzyme called primase, a
RNA-synthesizing enzyme that catalyzes the synthesis of short (6 – 11 bp) RNA primers required for
priming DNA synthesis. Primase works repeatedly on the lagging strand to synthesize the Okazaki
fragments.
3. List the enzymes that modify DNA and state their specific function
• Polymerase, catalyzes the formation of phosphodiester bonds
• Helicase, unwinds and untangles DNA
• Primase, synthesizes a short RNA primer to prime DNA synthesis
• Methyltransferase, adds a methyl group to a nitrogen base
• Deaminase, takes an amino group from a nitrogen base
• Ligase, catalyzes the formation of a single phosphodiester bond
• Protease, degrades proteins
• Ribonuclease, degrades RNA
• Exonuclease, remove bases from the ends of DNA strands
• Endonuclease, cut DNA strands internally
- Restriction enzymes → blunt and sticky ends
, - Star activity → altered specificity, binding and cutting sequences other than the
expected recognition sequences.
4. Describe the process of conjugation and 5. Compare transformation/transfection,
transduction and conjugation
Conjugation
Transduction
, Transformation
Transfer of
DNA from one
organism to
another
without
protection of a
conjugative
bridge or viral
coat.
*Transfection
in animal cells
Chapter 2: RNA
6. Compare and contrast the structure of RNA with that of DNA
- DNA has deoxyribose sugars; RNA has ribose sugars
- DNA has thymine; RNA contains uracil
- DNA is double stranded; RNA is single stranded
7. List and compare the different types of RNA present in the cell
- mRNA
- rRNA
- tRNA
- small nuclear RNA (functions in splicing)
- hnRNA
- miRNA
- siRNA
- dsRNA
8. Describe the process of transcription (RNA replication) and the functions of the enzymes
involved and compare it to DNA replication (eukaryote)
mRNA carries the information in DNA to the ribosomes, where it is translated into proteins. RNA
transcription is catalyzed by RNA polymerase (interphase).
- Works more slowly than DNA polymerase
- Less fidelity
- Doesn’t require priming
- Polymerized in 5’ to 3’ direction, the first ribonucleoside triphosphate retains all
of its phosphate groups and subsequent ribonucleoside triphosphate retain only
alpha phosphate (closest to ribose sugar).
- Three types of RNA polymerases in eukaryotes (one in prokaryotes).
• RNA polymerase I → synthesize noncoding RNA in nucleolus
• RNA polymerase II → synthesizes mRNA in the nucleus
• RNA polymerase III → synthesize noncoding RNA in the nucleus
RNA polymerase starts at the promoter (initiation) using one of the DNA strands as a template, only
replacing the A-T bond for a A-U bond (elongation). This proceeds until a polyadenylation signal
(polyA site) is encountered. As the polymerase proceeds past the polyA site, the nascent mRNA is
released by an endonuclease associated with carboxy terminal end of pol II.
- RNA synthesis after this part is bound by exonuclease and degraded
Les avantages d'acheter des résumés chez Stuvia:
Qualité garantie par les avis des clients
Les clients de Stuvia ont évalués plus de 700 000 résumés. C'est comme ça que vous savez que vous achetez les meilleurs documents.
L’achat facile et rapide
Vous pouvez payer rapidement avec iDeal, carte de crédit ou Stuvia-crédit pour les résumés. Il n'y a pas d'adhésion nécessaire.
Focus sur l’essentiel
Vos camarades écrivent eux-mêmes les notes d’étude, c’est pourquoi les documents sont toujours fiables et à jour. Cela garantit que vous arrivez rapidement au coeur du matériel.
Foire aux questions
Qu'est-ce que j'obtiens en achetant ce document ?
Vous obtenez un PDF, disponible immédiatement après votre achat. Le document acheté est accessible à tout moment, n'importe où et indéfiniment via votre profil.
Garantie de remboursement : comment ça marche ?
Notre garantie de satisfaction garantit que vous trouverez toujours un document d'étude qui vous convient. Vous remplissez un formulaire et notre équipe du service client s'occupe du reste.
Auprès de qui est-ce que j'achète ce résumé ?
Stuvia est une place de marché. Alors, vous n'achetez donc pas ce document chez nous, mais auprès du vendeur annabellewiegers. Stuvia facilite les paiements au vendeur.
Est-ce que j'aurai un abonnement?
Non, vous n'achetez ce résumé que pour €4,99. Vous n'êtes lié à rien après votre achat.
Peut-on faire confiance à Stuvia ?
4.6 étoiles sur Google & Trustpilot (+1000 avis)
71498 résumés ont été vendus ces 30 derniers jours
Fondée en 2010, la référence pour acheter des résumés depuis déjà 14 ans