Illumina - Guides d'étude, Notes de cours & Résumés

This is a summary of the course Genomics for Health and Environment (NWI-BB086). It contains information from the lectures, but also included images for better visualization. Good luck!

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AAB MT Molecular Diagnostic Next Generation 2023This technique uses polymerase to amplify nucleotides to form DNA clusters? Illumina Srquencing What technique detects sequencing by detection hydrogen ions that are released during the polymerization of DNA? IonTorrent Sequencing What next generation sequences DNA by ligation? SOLiD Sequencing This method uses ddNTPs that are flourescently labeled and does not use a primer? Dye Terminator During this technique each ...

Next Generation DNA Sequencing Exam Questions and Answers Graded A+ NGS Background - Answers 1990 - Lynx Therapeutics Massively Parallel Signature Sequencing (MPSS)->biggest revolution in molecular biology after sanger sequencing and PCR First Generation Sequencing (FGS) - Answers A low-throughput sequencing method that allows sequencing of a specific region of the genome (Sanger Method), low error rate, easy to anylyze-can do manually Next Generation Sequencing (NGS) - Answers A high-thro...

BIOMG 3320 Exam Questions With Correct Answers Bond between phosphate and sugar? - answerphosphodiester Bond between sugar and base? - answerglycosidic Non-covalent interactions? - answerH bonding, base stacking, electrostatic interactions Chargaff rules? - answerpurine content = pyrimidine content adenine = thymine guanine = cytosine Do base compositions differ between species? - answerYes - have different ratios Bacterial genome? _ micrometer, _______ DNA molecule, _____ million base ...

MB (ASCP) Review & Exam Questions Part II Questions and answers 2023 What is the Beer-Lambert Law? the linear relationship between absorbance and concentration of an absorbing species. What is the Beer-Lambert Law equation? A = ebc A: absorbance e: molar absorptivity c: concentration b: path length DNA and RNA are absorbed at: 260nm Proteins are absorbed at: 280nm What is the melting temperature equation? Tm = (4 x # of GC pairs) + (2 x # of AT p...

Advancements in DNA Sequencing Technologies Exam Questions and Answers Latest Update Sanger Sequencing - Answers First generation DNA sequencing method developed by Frederick Sanger. Next Generation Sequencing (NGS) - Answers Advanced sequencing technologies allowing massive parallel sequencing. Chain-Termination Method - Answers Technique using ddNTPs to stop DNA strand extension. Phi X 174 - Answers First genome sequenced using Sanger method in 1977. Dideoxyribonucleotide (ddNTP) - Answe...

MB ASCP Review and Exam Questions Part II (Already Graded A+ 2023) This analytical test amplifies the same target in two PCR reactions and uses the product of round 1 as the template for round 2. So there are 2 pairs of primers and 2 successive reactions. Basically you pre-amplify a broad region and then amplify a specific region. This test is good to use if you have low amounts of target because it increases specificity/sensitivity. - Answer Nested PCR This analytical test has multiple pai...

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DNA Sequencing and Genomics Vocabulary Exam Questions and Answers Dideoxynuceotide - Answers used in sanger sequencing also can called: ddNTP, ddATP, ddTTP, ddGTP, ddCTP lacks a 3'hydroxyl group so it cannot be elongated by DNA polymerase Sanger Sequencing - Answers most widely used sequencing method based on the selective incorporation of didoxynuceotides by DNA polymerase during in vitro DNA replication Illumina sequencing - Answers allows you to get billions of bases generates bill...