Golden gate cloning - Guides d'étude, Notes de cours & Résumés

Uitwerking van alle examenvragen uit de lijst op canvas gegeven door professor Van der Henst (2021). DISCLAIMER: De lijst met examenvragen varieert ieder jaar een beetje (hangt van gastsprekers af), maar het grootste deel blijft wel altijd hetzelfde. Part 0: Introduction: Prokaryoten Part 1: Prokaryoten: Plasmids and chromosome, selection and counter-selection markers Part 2: Prokaryoten: Gene deletion and cloning techniques Part 3: Promoters, reporter genes and PCR. Part 4: Natural mu...

A detailed explanation of how and why Golden Gate Cloning is conducted. Created by a graduate Biochemistry student who is now studying Biological Sciences at Cambridge University. This is designed to be used for all levels of learning, from GCSE to university. When learning biology at school, I often found that when some of the topics were too simplified I did not understand the information. Hence, I have created this document to be easy to understand but still fully explain key concepts.