Nested pcr - Guides d'étude, Notes de cours & Résumés

MB - ASCP Review Exam With Correct Questions and Answers Updated 2024 A+ Graded 100%. Her2/neu exhibits an overexpression, what would this indicate? - ANSWER-More aggressive growth and metastasis of the tumor cells in human breast cancer. Predominant form of DNA - ANSWER-Right-handed B DNA and histone interaction is due to what type of bond? - ANSWER-Ionic What stains to the minor groove of DNA duplex? - ANSWER-SYBR Green What is the most abundant type of RNA? - ANSWER-Ribosomal RN...

ASCP MB Exam Questions & Answers 2024 Which PCR method has the highest specificity? A. qPCR B. Touchdown PCR C. Nested PCR D. Two-step PCR - ANSWER-Nested PCR Given the anti-sense strand of DNA: 5'-AATTGCCGACATAGAT-3' which is the appropriate sense strand? A. 5'-ATCTATGTCGGCAATT-3' B. 5'-TTAACGGCTGTATCTA-3' C. 5'-AATTGCCGACATAGAT-3' D. 5'-GAGCACGCTATCTTAT-3' - ANSWER-5'-ATCTATGTCGGCAATT-3' During DNA replication, the 3' -OH of the growing DNA chain attacks wh...

Molecular Diagnostics Final (Exam + Quiz MC) /verified/ d (primers) *Ans* *The specificity of the PCR reaction is determined by which of the following components?* a. Mg2+ concentration b. Deoxynucleotides c. DNA polymerase d. Primers a (primer dimers) *Ans* *Complementarity of primer pairs at the 3' ends will result in what artifacts?* a. Primer dimers b. Large mis-primed products c. No amplification d. 5' end degradation of the primers d (secondary structure) *Ans* *A DNA se...

B form DNA correct answersRight handed, 10.5 bp Lysine (K) and Arginine (R) correct answersAssociated with Histones Bromophenol blue correct answersTracking dye Capillary electrophoresis correct answersMust be ss PFGE buffer correct answersTBE Store RNA > 7 months correct answers-70 ethanol Branched DNA correct answersDNA/RNA targets Methylation or FISH correct answersPWS/AG Nested PCR correct answers2nd step, more specific Denaturation HPLC correct answersDetecting...

MB (ASCP) 2020(updated) correctly answeredConsider the table below where a child and a possible father (PF) share the listed paternity indices for each locus listed (LOC-A1, LOC-B2, LOC-C3, LOC-D4). Locus Tested PF Child Paternity Index LOC-A1 3 2/3 2.18 LOC-B2 7/5 5 0.798 LOC-C3 15/17 9/17 5.21 LOC-D4 12 12 1.37 Based on the data presented in the table, what is the combined paternity index, CPI, from the loci tested: LOC-A1, LOC-B2, LOC-C3 and LOC-D4? 12.42 9.558 15.56 2.38 12.42...

d (primers) - Answer *The specificity of the PCR reaction is determined by which of the following components?* a. Mg2+ concentration b. Deoxynucleotides c. DNA polymerase d. Primers a (primer dimers) - Answer *Complementarity of primer pairs at the 3' ends will result in what artifacts?* a. Primer dimers b. Large mis-primed products c. No amplification d. 5' end degradation of the primers d (secondary structure) - Answer *A DNA sequence with high GC content is more likel...

MB (ASCP) Exam questions and answers latest 2024 Predominant form of DNA - -Right-handed B DNA and histone interaction is due to what type of bond? - -Ionic What stains to the minor groove of DNA duplex? - -SYBR Green What is the most abundant type of RNA? - -Ribosomal RNA (rRNA) What does RNA act as in a retrovirus? - -Genome and mRNA What increases half-life of mRNA? - -5' Methyl Cap What kind of DNA is most likely to be transcriptionally active? - -Euchromatin What is the most common...

MB ASCP Exam QUESTIONS & ANSWERS 2023 (A+ GRADED 100% VERIFIED) 1. Which of the following is not a common hydrogen bond acceptor? A. A carbonyl B. Primary amine C. Hydroxyl D. Tertiary amine ~ Answer: Primary amine 2. How is warfarin dosage affected in patients with VKORC1 deficiency? A. Patients receive a higher warfarin dose B. Patients receive a lower warfarin dose C. Warfarin dosage is unaffected ~ Answer: Patients receive a lower warfarin dose 3. You co...

The specificity of the PCR reaction is determined by which of the following components? - Answer Primers Complementarity of primer pairs at the 3′ ends will result in what artifacts? - Answer Primer dimers A DNA sequence with high GC content is more likely to have what property that interferes with PCR amplification? - Answer Secondary structure What is the advantage of a nested PCR procedure? - Answer Nested PCR offers increased specificity and yield of product. Real-time ...

MB (ASCP) Practice Exam 2024 100% Correct What does barcoding do in Next Generation Sequencing? - Answer ️️ -Adds unique tag to all nucleic acids within one sample so that various samples can be mixed What is the best way to store RNA for longer than a month? - Answer ️️ -In Ethanol, at -20°C or -80°C Testing for Aldehyde Dehydrogenase deficiency is important in patients with: - Answer ️️ - Alcohol dependence During DNA replication, the 3' -OH of the growing DNA chain attac...