Histotechnology: Decalcification and Processing verified to pass

Histotechnology: Decalcification and Processing After completion of decalcification, the specimen should be - correct answers Washed in water Over decalcification is more noticeable in staining of nuclei or cytoplasm? - correct answers Nuclei Acid solutions soften bone by removing what type of salts? - correct answers Calcium salts What type of acid is a chelating agent used in decalcification? - correct answers EDTA (ethylene diamine tetra acetic acid) To offset the hydrolysis of nucleic acids caused by decalcification, bone marrow is often fixed in what solution? - correct answers Zenker's During decalcification, what type of gas is released? - correct answers Carbon dioxide What four factors influence the rate of decalcification? - correct answers Size of specimen, concentration of decal solution, Time in decal solution Amount of decal solution For proper decalcification, bone should be cut at no more than - correct answers 4-5 mm thick Chelating agents act by binding to: - correct answers Calcium ions Four methods of decalcification are: - correct answers Acid, Ion exchange, Electrolytic method, Chelation