100% tevredenheidsgarantie Direct beschikbaar na betaling Zowel online als in PDF Je zit nergens aan vast
Eerder door jou gezocht
BIO 353 Study Guide exam 1 1. The cell: The basic unit of structure and function in living things 2. What are the major Organelles/ structures of a basic eukaryotic cell?: nucleus nucleolus ribosomes smooth ER rough ER mitochondria 3. What are th€10,87
In winkelwagen
BIO 353 Study Guide exam 1 1. The cell: The basic unit of structure and function in living things 2. What are the major Organelles/ structures of a basic eukaryotic cell?: nucleus nucleolus ribosomes smooth ER rough ER mitochondria 3. What are th
2 keer bekeken 0 keer verkocht
Vak
BIO353
Instelling
BIO353
BIO 353 Study Guide exam 1
1. The cell: The basic unit of structure and function in living things
2. What are the major Organelles/ structures of a basic eukaryotic cell?: nucleus
nucleolus
ribosomes
smooth ER
rough ER
mitochondria
3. What are th
1. The cell: The basic unit of structure and function in living things
2. WHAT ARE THE MAJOR ORGANELLES/ STRUCTURES OF A BASIC EUKARYOTIC CELL?: NUCLE-US
nucleolus
Aribosomes
smooth ER
rough ER
mitochondria
3. What are the 3 major disciplines involved with studying biology?: bioimaging
biochemistry
mol. biology/genetics
4. What is light microscopy?: visible light passes through a series of lenses to
produce a magnified image
5. WHAT IS THE RESOLUTION LIMIT OF THE LIGHT MICROSCOPE?: .2 NM
6. Define resolution: Describes the degree of clarity, detail, and sharpness of an
image
7. Define magnification: the ratio of an object's image size to its real size
8. what is one of the major advantages of the basic light microscope?: ability
to image LIVE cells
9. STATE OF CELLS WHEN USING AMPLITUDE CONTRAST: FIXED/DEAD
10. Describe amplitude contrast: a form of contrast generated by the absorptionof
selective wave lengths from the visible light spectrum
11. Describe phase contrast microscopy: dependent on light refraction
contrast formed b/c different parts have different densities
12. STATE OF CELLS WHEN USING PHASE CONTRAST ?: LIVING
13. epifluorescence microscopy: detection of specific molecules/ions/organelles
that are MADE fluorescent by coupling them to a flourochrome marker
14. indirect immunofluorescence: i. cells/tissues fixed
ii. exposed to primary antibody, antibody binds to specific protein (ie antigen)
iii. exposed to secondary antibody that is coupled to a flourochrome marker
, BIO 353 Study Guide exam
1
15. What is GFP and how is it coupled to the proteins in different cellular
structures (direct method)?: Green Fluorescent Protein binds the flourescent
sntibody to the corresponding antigen on the target protein
16. Whats the advantage of using GFP?: cells are ALIVE
17. What are vital fluorescent dyes (direct method)?: harmless dyes used to
stain living tissue for observation
18. confocal microscopy: a light microscope that uses fluorescent stains and laserto
make two- and three-dimensional images
, BIO 353 Study Guide exam
1
19. Advantages of confocal microscopy: increased clarity of images and resolu-
tion
20. What is electron microscopy?: technique used for obtaining high resolution
images of specimens
21. transmission electron microscope: used to study the internal structure of cells
22. Advantage of electron microscope: shorter wavelength of electron beam=
increased resolution and magnification
23. Disadvantage of electron microscopes: cannot view living things- life is not
compatible with vacuum
24. Electron tomography: provides highest and most accurate 3-D resolution for
ultrasound studies
25. scanning electron microscopy: beams scan across and build image layer bylayer
**EXTERNAL STURCTURES
26. what are the 4 common procedures for homogenizing cells?: 1. high fre-
quency sound
2. mild detergent
3. force cells through small pore using high pressure
4. shear cells between a close fitting rotating plunger and wall of glass vessel
27. Centrifugation: Separates subcellular structures and macromolecules into dif-
ferent parts (fractions) by size, shape, and density
28. differential centrifugation: uses progressively higher speeds to separate the
cellular components by size and density
29. density gradient centrifugation: A method of separating particles by centrifu-
gation through a gradient of a dense substance, such as sucrose or cesium chloride.
** based on buoyant density
30. what are the characteristics that can vary between proteins?: charge,
shape, size
31. one dimensional sds page: separates peptides based on SIZE (MASS)
32. describe methods of 1d sds page: 1. SDS coats proteins in negative charge
2. proteins migrate
3. SDS+ heat= proteins unfold
33. 2-D SDS PAGE: native proteins are separated based on CHARGE usingiso-
electric focusing (first dimension) and SIZE (mass) in second dimension)
34. Western blot: identifies peptides
Voordelen van het kopen van samenvattingen bij Stuvia op een rij:
√ Verzekerd van kwaliteit door reviews
Stuvia-klanten hebben meer dan 700.000 samenvattingen beoordeeld. Zo weet je zeker dat je de beste documenten koopt!
Snel en makkelijk kopen
Je betaalt supersnel en eenmalig met iDeal, Bancontact of creditcard voor de samenvatting. Zonder lidmaatschap.
Focus op de essentie
Samenvattingen worden geschreven voor en door anderen. Daarom zijn de samenvattingen altijd betrouwbaar en actueel. Zo kom je snel tot de kern!
Veelgestelde vragen
Wat krijg ik als ik dit document koop?
Je krijgt een PDF, die direct beschikbaar is na je aankoop. Het gekochte document is altijd, overal en oneindig toegankelijk via je profiel.
Tevredenheidsgarantie: hoe werkt dat?
Onze tevredenheidsgarantie zorgt ervoor dat je altijd een studiedocument vindt dat goed bij je past. Je vult een formulier in en onze klantenservice regelt de rest.
Van wie koop ik deze samenvatting?
Stuvia is een marktplaats, je koop dit document dus niet van ons, maar van verkoper Millard. Stuvia faciliteert de betaling aan de verkoper.
Zit ik meteen vast aan een abonnement?
Nee, je koopt alleen deze samenvatting voor €10,87. Je zit daarna nergens aan vast.
