Microbial Growth and Aerotolerance questions and answers

bacterial colony - Arises from a single cell - Represents a group of bacterial cells that are the genetically the same isolated colonies separated from each other quadrant streak used to obtain isolated colonies (individual species) to generate a pure culture MacConkey Agar MAC MacConkey Agar used as a selective and differential medium MacConkey Agar bile salts and crystal violet inhibit the growth of gram positive organism, lactose provides a source of fermentable carbohydrates allowing for differentiation MacConkey Agar neutral red is a pH indicator that turns red at a pH below 6.8 and is colorless at any pH greater than 6.8 MacConkey Agar ph below 6.8 red MacConkey Agar ph above 6.8 colorless Mannitol Salt Agar MSA Mannitol Salt Agar determines if the bacteria are halophilic and can ferment mannitol halophilic salt loving Mannitol Salt Agar if the bacteria are halophilic then it is halophilic because of its ability to grow in the high salt environment Mannitol Salt Agar positive result yellow halo around the bacte