Lecture 22 Protein Engineering Exam Questions With Verified Answers.

Lecture 22 Protein Engineering Exam Questions With Verified Answers. What is protein engineering? - answermodification of a protein with recombinant DNA technology to get a better use in medicine Enzymes work more efficiently because they are __________ in harsh conditions. - answerstable General Methods for Gene Mutation: Method 1 - Classic Mutagenesis by Radiation: UV lights cross-link two neighboring ________________ bases in DNA. Errors and mutations are introduced during DNA replication and repaired by host enzymes. - answerpyrimidine What is the limitation to Method 1-Classic Mutagenesis by Radiation? - answerInefficient, out of control General Methods for Gene Mutation: Method 2 - Site-directed mutagenesis: PCR-amplified DNA is or is not methylated? - answeris NOT Method 2 - Site-directed mutagenesis: Plasma isolated from E. Coli cells are or are not hypermethylated? - answerare What is the function of Dpnl enzyme? - answerdigests methylated DNA What is the limitation of Method 2 - Site-directed mutagenesis? - answera limited scope (how do you remove the parent template?) General Methods for Gene Mutation: Method 3 - Error-prone PCR: PCR conditions are changed to make it prone to errors during amplification. Ther