Site directed mutagenesis - Samenvattingen, Aantekeningen en Examens

______ methodology allows for the study of complex *cellular regulation of genes *the role of specific amino acid residues in protein structure, catalytic function and ligand binding capacity *mechanisms involved in infectious and genetic disease - Answer Site directed mutagenesis

What describes a well defined PCR reaction? - Answer - template DNA is added in purified form - typically starts with 10^5 - 10^6 molecules of template DNA What are essential components to site directed mutagenesis? - Answer - DNA template - Primers - dNTPs - DNA polymerase

How does CRISPR work naturally? Correct Answer Clustered Regularly Interspaced Short Palindromic Repeats - bacterial defense mechanism - bacteria have short palindromic repeats separated by spacer regions which are segments of DNA from past viral exposure - when attacked again by same virus, the spacer region is used as a template and the guide RNA (tracrRNA) helps recognize the viral DNA and degrade it using Cas9 nuclease at the specific site - need PAM (helps distinguish between self and n...

Lecture 22 Protein Engineering Exam Questions With Verified Answers. What is protein engineering? - answermodification of a protein with recombinant DNA technology to get a better use in medicine Enzymes work more efficiently because they are __________ in harsh conditions. - answerstable General Methods for Gene Mutation: Method 1 - Classic Mutagenesis by Radiation: UV lights cross-link two neighboring ________________ bases in DNA. Errors and mutations are introduced during DNA replica...

Protein Engineering Exam Questions With Verified Answers. Why create diversity? - answer•Control evolution -Speeding up the natural randomness -Focusing changes •Refine creations -Post screening enhancements -Locating unknown fitness apex Random mutagenesis (PCR-based) - answerRequirements: •Low-fidelity polymerase •Degenerative primers •"Mimics" nature •No knowledge needed! Random mutagenesis (non-PCR-based) - answer•Transposons insert themselves within DNA -Random...

PCR allows for: amplification of minute amounts of template DNA can generate thousands or millions of copies of that sequence What is one of the most important parameters for a successful PCR? well designed primers Previous Play Next Rewind 10 seconds Move forward 10 seconds Unmute 0:00 / 0:15 Full screen Brainpower Read More Name 3 variables that influence the success of a PCR reaction 1. quality of template DNA 2. cycling conditions 3. specificity and stabilit...

MICR 351 TESTED QUESTIONS WITH ALL ANSWERS AND ALL PRINTED PAGES Northern Hybridization? - Answer-mRNA-cDNA hybridization to find gene of interest using DNA probe to identify which bond holds gene of interest Western Hybridization? - Answer-Antibody-protein hybridization, where antibodies bind to a specific protein bond that contains target protein that produces antibody - used in vaccines and other tests, such as pregnancy tests Functional genomics - Answer-Microarrays and RNA-sequence...

The success of a PCR reaction is mostly dependent on what? well designed primers Primer design can also be applied to what? - probes in Southern and Northern blots - DNA sequencing - microarray design Previous Play Next Rewind 10 seconds Move forward 10 seconds Unmute 0:00 / 0:15 Full screen Brainpower Read More What else can affect the success of a PCR reaction? - Quality of the template DNA - cycling conditions - Specificity and stability of the PCR primers ...

what variables are considered in primer design - length, sequence, %GC, Tm, Ta, 3'end stability, 5' end stability, secondary structures why is the formation of secondary structures in primers especially undesirable - reduces primer available to use to make product standard denaturation temp and time (initial and denaturation) - 94C 1-5min initial 20-45 sec std annealing temp and time - temp varies between protocols, 30s std elongation temp and time - temp depends on polymerase u...

GCD Quiz 7/8 Practice 69 Questions with Verified Answers Which of the following may be used as a vector in a gene-cloning experiment? a.mRNA b.Plasmid c.Virus d.Both b and c - CORRECT ANSWER d The restriction enzymes used in gene-cloning experiments _______, which generates sticky ends that can _______. the DNA, enter bacterial cells the DNA, hydrogen bond with complementary sticky ends late DNA, enter bacterial cells late DNA, hydrogen bond with complementary sticky ends - CORRE...