AA 1

A technician investigated the effect of temperature on the rate of an enzyme-controlled reaction. At each temperature, he started the reaction using the same concentration of substrate. Figure 1 shows his results. Give two other factors the technician would have controlled. [1 mark] Draw a tangent on each curve to find the initial rates of reaction. Use these values to calculate the ratio of the initial rates of reaction at 60 °C : 37 ° C. Show your working. [2 marks] 0 1 . 2 0 ...