BIOL 1510 The Properties of Enzymes Lab Report (Biology)

Extra credit assignment for BIOL 1510. Honeypot ant

In part one of the lab the optimum pH of an enzyme was tested using ALP. To begin, 6.5mL of Solution B and 6.5mL of dH2O were added to Solution E. Four cuvettes were labeled one through four. The spectrophotometer was then set at 405 nm. In the first cuvette, which was the control, 3mL of solution E were added, along with 2 mL of dH2O to make up a total volume of 5mL. In the second acidic cuvette, 3 mL of solution E were added, along with 1.9mL of 0.2 M HCl, and 100 uL of high conc. enzyme ...