BSCI 222

These lecture notes cover Topic 8, focusing on induced mutations, DNA repair mechanisms, and genetic regulation in prokaryotic and eukaryotic organisms. The notes provide an overview of various types of mutations, their phenotypic effects, and the consequences of mutations on functional sequences. Additionally, the document discusses DNA repair mechanisms such as homologous recombination repair (HRR) and nonhomologous end joining (NHEJ), as well as chromosomal variations and transposable element...

These lecture notes cover Topic 12, focusing on RNA-based regulation and its effects on gene expression. The notes provide a comprehensive overview of various RNA regulatory mechanisms in both prokaryotes and eukaryotes, including transcription, translation, and chromatin structure changes. Key topics include mRNA secondary structures, riboswitches, antisense RNA, RNA interference (RNAi), and long non-coding RNAs (lncRNAs). Additionally, the notes explore specific examples and experimental appli...

These lecture notes cover Topic 13, focusing on the fundamentals of genomics. The notes explain the genome sequencing, from collecting tissue samples and extracting DNA to sequencing using Illumina technology. They detail the steps involved in aligning reads, forming contigs, and improving genome assemblies with genetic maps. Additionally, they explore gene annotation and the role of bioinformatics in genome assembly and annotation, providing a comprehensive overview of what a sequenced genome l...