HTL ASCP

Acetic Acid - Answer Non coagulant, penetrates quickly and leaves tissues very soft. Used often as an additive to precipitate and preserve the nucleoproteins and fix the nuclei. Acetic Acid - Answer Swells tissues dramatically by breaking collagen bonds and allowing bonds to absorb water. Used as additive to counter-act the shrinking effect of other fixatives. Formaldehyde - Answer Non coagulant and additive fixative that reacts with amino groups that link protein chains together....

Cresyl Echt Violet (Luna) method - Answer Nissl substances (basophilic) dyed, alcohol used to differentiate. Cresyl Echt Violet (Vacca) method - Answer Nissl substances (basophilic) dyed, acetic acid used to differentiate. Bodain Method for Nerve Fibers - Answer Protargol (silver solution) with added COPPER destains connective tissue. Hydroquinone reduces, gold chloride, sodium thiosulfate follows.

Polarizing Microscope - Answer Microscope used to identify crystals, talc, silica, or urates. Birefringence - Answer Transmitting light unequally in different directions- Phase-Contrast Microscope - Answer Microscope used to examine unstained objects. Not routinely used in histopath labs.

Dehydrating Agents - Answer Alcohol, dioxane, acetone, tetrahydrofuran. Clearing Agents - Answer Xylene, toluene, benzene, acetone, chloroform, cedarwood oil, tetrahydrofuran, dioxane, limonene, aliphatic hydrocarbons. Universal Solvent - Answer Solvents that perform both the dehydration and clearing steps. Example Dioxane, Tetrahydrofuran.