Cell Free Gene Expression

CFE systems offer barrier-free control over biological processes to directly probe function, making it an attractive approach for prototyping genetic parts and designs as well as a suite of point-of-use applications. Direct access to the reaction environment, however, comes with a degree of variability with and sensitivity to added components. Thus, having quality control standards for reagents is essential. With this in mind, Chapter 1 offers a suite of tactics for DNA handling and reprodu...