Affinity chromatography - Study guides, Class notes & Summaries

History of chromatography (ch 23) - correct answers -Greek word "chromatus" for color -Tswett (1903) first separated plant pigments Applications - correct answers Breast milk, water analysis, urinalysis for drug doping Goals of separation - correct answers Recovery of product, purity of product Type, SP, MP - correct answers 1. Adsorption: one polar substance sticks to another polar substance. Solid SP, gas or liquid MP 2. Partition: dissolving of 2 similar liquids. Liquid SP, gas o...

Henderson-Hasselbach Equation - pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography - Separates sample based on size with smaller molecules eluting later. Ion-Exchange...

Goals of Separation - correct answers recovery of product, purity of product Types of Chromatography - correct answers Adsorption - SP: solid, MP: gas/liquid Partitioning - SP: liquid, MP: gas/liquid Ion-Exchange - SP: bonded anion/cation, MP: liquid Molecular Exclusion - SP: has pores, MP: liquid Affinity - SP: antigen/antibody interactions, MP: liquid Electrophoretic - no SP Solvent Extractions based on - correct answers Some solvents are not miscible; "like dissolves like"; less ...

ACS BIOCHEMISTRY EXAM QUESTION WITH COMPLETE SOLUTIONS LATEST 2023/2024 COMPLETE SOLUTIONS LATEST 2023/2024 Henderson-Hasselbach Equation - ANSWER pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - ANSWER Use d in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - ANSWER Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solu...

Henderson-Hasselbach Equation - pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography - Separates sample based on size with smaller molecules eluting later. Ion-Exchange...

The study of chemical drugs, their chemistry, effects on the body, actions, toxicities, and dosages includes research into newly discovered chemicals - Pharmacology The science encompassing those phases of knowledge relating to natural products that are generally of medicinal value and primarily of plant origin. - Pharmacognosy Science of preparing, compounding, dispensing herbal or chemical medicines. - Pharmacy Used with herbs of potential toxicity, whereby the amount of liquid herb per d...

Goals of separation - correct answers Recovery of product- catching the desired solute after separation Purity of product- how many and how much of various components Type of Chromatography - correct answers Partioning= liquid SP, liquid or gas MP Adsorption (Chemical sticking)- solid SP, liquid or gas MP Ion-Exchange- bonded anion(-) or action (+) SP, liquid MP Molecular Exclusion (size exclusion, gel permeation)-pores SP, liquid MP Affinity- Antigen/Antibody SP, liquid MP Solvent do...

ACS BIOCHEMISTRY EXAM QUESTION WITH COMPLETE SOLUTIONS LATEST 2024. Henderson-Hasselbach Equation - CORRECT ANSWER - pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - CORRECT ANSWER - Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the Nterminus. Salting Out (Purification) - CORRECT ANSWER - Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution....

ACS BIOCHEMISTRY Solved Exam |100% Pass| Henderson-Hasselbach Equation *Ans* pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis *Ans* Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) *Ans* Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography *Ans* Separates sample based on ...

ACS BIOCHEMISTRY EXAM QUESTION WITH COMPLETE SOLUTIONS LATEST 2024. Henderson-Hasselbach Equation - CORRECT ANSWER - pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - CORRECT ANSWER - Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the Nterminus. Salting Out (Purification) - CORRECT ANSWER - Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. ...