Bcmb 406b - Study guides, Class notes & Summaries

BCMB 406B Midterm 1 Test Latest Update ...

what two criteria need to be meet when picking a replacement residue in SDM - Answer remove charge of functional group of original residue be similar or smaller in size What are the most significant problems in primer design - Answer secondary structures (loses primer), 3' end stability (mispriming), Tm difference (hard to set up) Primer length - Answer 18-22bp

PCR allows for: amplification of minute amounts of template DNA can generate thousands or millions of copies of that sequence What is one of the most important parameters for a successful PCR? well designed primers Previous Play Next Rewind 10 seconds Move forward 10 seconds Unmute 0:00 / 0:15 Full screen Brainpower Read More Name 3 variables that influence the success of a PCR reaction 1. quality of template DNA 2. cycling conditions 3. specificity and stabilit...

BCMB 406B Midterm 1 Lab 1 Test Latest Update ...

what variables are considered in primer design - length, sequence, %GC, Tm, Ta, 3'end stability, 5' end stability, secondary structures why is the formation of secondary structures in primers especially undesirable - reduces primer available to use to make product standard denaturation temp and time (initial and denaturation) - 94C 1-5min initial 20-45 sec std annealing temp and time - temp varies between protocols, 30s std elongation temp and time - temp depends on polymerase u...

BCMB 406B - Lab 1 Test With Complete Solution ...

BCMB 406B - Lab 2 Test Study Guide ...

0.1 ng vs. 1 ng pET28a w/ Kan selection - Answer - Showed that the amount of pET28a plasmid DNA doesn't affect the transformation efficiency 0.1 ng vs 0.1 ng (1/10 dilution) pUC19 w/ Kan selection - Answer - Showed that the amount of pUC19 present influenced the transformation efficiency Untreated CBM26-3 PCR product - Answer - Shows that the WT DNA can be transformed and grow on a plate without the DpnI treatment