Frnsc 100 exam 1 - Study guides, Class notes & Summaries

FRNSC 420 - EXAM 4 PRACTICE QUESTIONS AND ANSWERS (100% PASS) positive allosteric regulation - Answer️️ -binding of a regulatory molecule to an enzyme induces a conformation change, increasing the enzyme's affinity for a substrate - proteins are activators = enhance RNAP binding and/or induce conformational change, can change binding constant (KB) or rate constant (kf), lowers activation energies negative allosteric regulation - Answer️️ -bound regulatory molecule must release ...

FRNSC 420 - EXAM 2 PRACTICE QUESTIONS AND ANSWERS (100% PASS) nucleosome assembly/reassembly after replication - Answer️️ -- old H3-H4 tetramers remain bound to one of two daughter duplexes at random - old H2A-H2B dimers dissociate and compete to associate with tetramers - DNA sliding clamps mark replicating DNA and recruit chromosome assembly factors (CAFs) as chaperones - CAFs free nucleosomes and escort to newly synthesized DNA; NAP-1 escorts new/old H2A-H2B dimers, CAF-1 escorts ...

FRNSC 420 - EXAM 3 PRACTICE QUESTIONS AND ANSWERS (100% PASS) electrophoretic mobility shift assay (EMSA) - Answer️️ -demonstrates if a protein is bound to DNA - fragment is placed in non-denaturing polyacrylamide gel (native PAG) to keep protein associated to DNA - DNA/protein complex moves much slower in the gel due to added molecular weight (an electrophoretic mobility shift) - will appear higher in the lane compared to free DNA - migration depends on mobility of the protein; dif...

FRNSC 421W - EXAM 1 PRACTICE QUESTIONS AND ANSWERS (100% PASS) RFLP - Answer️️ -restriction fragment length polymorphism - DNA digested by restriction enzymes at repeat sequences, yields different fragment lengths for different people - Gel electrophoresis to separate fragments by size, MW ladder for reference - Southern blotting to physically transfer DNA to membrane - Radioactively labeled probe hybridizes with immobilized DNA on membrane - Exposure to X-ray film yields carbon copy...

FRNSC 421W - EXAM 2 PRACTICE QUESTIONS AND ANSWERS (100% VERIFIED AND UPDATED) DNA mixtures - Answer️️ -mixed biological fluids/cells of any type - saliva & skin cells - blood & semen - urine from two sources - sexual assault cases often contain female epithelial/white blood cells & male sperm, epithelial/white blood cells characteristics of sperm - Answer️️ -- condensed nucleus in the head, encapsulated by acrosome - mid-piece with ~50 (range from 10-1300) copies of mitochond...

FRNSC 421 - EXAM 3 PRACTICE QUESTIONS AND ANSWERS (100% PASS) lineage markers - Answer️️ -useful for assisting with the identification of related individuals separated by generational events - Y DNA paternally inherited - mtDNA maternally inherited Thomas Jefferson case - Answer️️ -Y-STR analysis on descendent of Eston Hemmings to determine if Thomas Jefferson had an affair with his slave Sally Hemmings - showed that Eston is related to the Jefferson family, cannot conclusively s...