Isoelectric point pi - Study guides, Class notes & Summaries

MCAT - Lab Techniques (MilesDown)Native-PAGE - correct answer A polyacrylamide gel electrophoresis method for proteins using NON-DENATURING conditions. Proteins keep their native charge and structure so they are separated based on charge and size. SDS-PAGE - correct answer A polyacrylamide gel electrophoresis method for proteins using DENATURING conditions. Sodium Dodecyl Sulfate denatures the proteins and gives the proteins a uniform charge. This allows them to be separated solely on mass, t...

ACS Biochemistry First Semester Exam Questions and Verified Solutions 2024 Buffer Order and Henderson Hasselbach - Answer -Water, Buffer, Salt, Base pH = pKa + log(A/HA) pKa decrease as temp increase so pH decrease Ramachandran plots - Answer -Φ = N-R ψ = C-R optimal angle to prevent steric hinderance Protein structure of a helix, B sheet, reverse turn - Answer -a: LEMARKHQ B: VICYFTW RT: PSDNG how many amino acids in alpha turn helix - Answer -3.6 Super secondary structure - Answe...

MCAT - Lab Techniques (MilesDown) Native-PAGE - correct answer A polyacrylamide gel electrophoresis method for proteins using NON-DENATURING conditions. Proteins keep their native charge and structure so they are separated based on charge and size. SDS-PAGE - correct answer A polyacrylamide gel electrophoresis method for proteins using DENATURING conditions. Sodium Dodecyl Sulfate denatures the proteins and gives the proteins a uniform charge. This allows them to be separated solely on mass,...

BIOCHEM ACS STUDY GUIDE QUESTIONS AND ANSWERS 2024. Each of the following subcellular structures contains DNA EXCEPT A. the nucleus. B. ribosomes. C. chloroplasts. D. mitochondria. - ANSWER- B In plant and animal cells, the three-dimensional integrity of the cell is maintained, in part, by an internal network of filaments called A. the cell wall. B. the Golgi apparatus. C. the cytoskeleton. D. the endoplasmic reticulum. - ANSWER- C Of the following, which are prokaryotes? A. viruses...

BCH4024 TEST 1 QUESTIONS WITH COMPLETE SOLUTIONS Isoelectric Point of Amino Acids - Answer-At the isoelectric point (pI = pH), average charge equals 0 The Henderson-Hasselbalch Eqn. can be applied at pI for both equilibrium dissociations. Two dissociation reactions involve the net zero charge species HAH+ <--> HA + H+ HA <--> A- + H+ (Isoelectric Point of Amino Acids) Two Henderson-Hasselbalch equations can be summed to solve for pI. - Answer-2pI=pKa1+ pKa2 +log [HA] +log...

1. A 53-year-old woman is found, by arteriography, to have 50% narrowing of her left renal artery. What is the expected change in blood flow through the stenotic artery? Decrease to 1⁄16 2. When a person moves from a supine position to a standing position, which of the following compensatory changes occurs? Increased contractility 3. At which site is systolic blood pressure (blood pressure) the highest? Renal artery 4. A person's electrocardiogram (ECG) has no P wave, ...

Buffer Order and Henderson Hasselbach - Water, Buffer, Salt, Base pH = pKa + log(A/HA) pKa decrease as temp increase so pH decrease Ramachandran plots - Φ = N-R ψ = C-R optimal angle to prevent steric hinderance Protein structure of a helix, B sheet, reverse turn - a: LEMARKHQ B: VICYFTW RT: PSDNG how many amino acids in alpha turn helix - 3.6 Super secondary structure - helix turn helix parallel vs. antiparallel - parallel wedges face same way antiparallel face opposit...

pKa (chapter 3) correct answers measure of the tendency of a group to give up a proton (acidity); tendency decreases tenfold as pKa increases by one unit thioester bond (1) correct answers compounds with functional group C-S-CoA-C (eg, in acetyl-CoA) zwitterion (3) correct answers dipolar ion with both positive and negative groups but overall neutral charge; can act as either an acid or base Isoelectric point (pI) (chapter 3) correct answers the characteristic pH at which the net electr...

Buffer Order and Henderson Hasselbach correct answers Water, Buffer, Salt, Base pH = pKa + log(A/HA) pKa decrease as temp increase so pH decrease Ramachandran plots correct answers Φ = N-R ψ = C-R optimal angle to prevent steric hinderance Protein structure of a helix, B sheet, reverse turn correct answers a: LEMARKHQ B: VICYFTW RT: PSDNG how many amino acids in alpha turn helix correct answers 3.6 Super secondary structure correct answers helix turn helix parallel vs. ant...

ACS Gen Chem 1 Exam 48 Questions with Verified Answers Ion - CORRECT ANSWER A species of an element in which the number of electrons does not equal the number of protons. Isotope - CORRECT ANSWER Each isotope of an atom has a different number of neutrons. Isoelectric - CORRECT ANSWER Two atoms with the same charge. Isotopic - CORRECT ANSWER Two atoms with the same number of neutrons. Transition Metal Orbitals - CORRECT ANSWER The 4s orbital is filled before the 3d orbital, but lis...