Methylation of dna - Study guides, Class notes & Summaries

Describe the five general procedures in DNA cloning Correct Answer-1. Obtaining the DNA segment to be cloned 2. Selecting a small molecule of DNA capable of autonomous replication 3. Joining two DNA fragments covalently 4. Moving recombinant DNA from the test tube to a host organism 5. Selecting or identifying host cells that contain recombinant DNA Are restriction enzymes made by eukaryotes or prokaryotes? What is their function in vivo? Correct Answer-Restriction endonucleases are found...

NR 546 Midterm Exam 2023/2024 Questions AND Answers Update A+ What should the PMHNP consider when prescribing chemical restraints? - Answer-- allergy status -prior med hx for adverse drug reactions r/t the meds ordered in the chemical restraint -state regulations regarding chemical restrains must be reviewed Are the PMHNP and other staff liable if the client has an allergic reaction or adverse side effects to the drugs used for chemical restraint? - Answer-No. The client has been court-...

Test Bank for Huether and McCance's Understanding Pathophysiology, Canadian Edition, 2nd Edition by Kelly Power, Stephanie Zettel, , Mohamed Toufic El-Hussein Table of Contents PART ONE: BASIC CONCEPTS OF PATHOPHYSIOLOGY Unit 1: The Cell 1. Cellular Biology 2. Genes and Genetic Diseases 3. Epigenetics and Disease 4. Altered Cellular and Tissue Biology 5. Fluids and Electrolytes, Acids and Bases Unit 2: Mechanisms of Self-Defense 6. Innate Immunity: Inflammation and Wound Healing ...

Histones are rich in what AAs? - ANSWER ☑☑Lysine and arginine (both + charge) When does histone synthesis occur? - ANSWER ☑☑S phase (along with DNA getting synthesized) Heterochromatin is _________, euchromatin is _________ - ANSWER ☑☑Inactive/condensed; active/accessible What allows mismatch repair enzymes to distinguish between old and new DNA strands in prokaryotes? - ANSWER ☑☑Methylation; old/template strands are methylated, new/daughter strands are unmethylated ...

Stahl's Essential Psychopharmacology Comprehensive Exam (2024) || Questions & Answers (100% Verified) Stahl's Essential Psychopharmacology Comprehensive Exam (2024) || Questions & Answers (100% Verified) MEK does what? - ANSWER - Activates ERK (extracellular signal-regulated kinase), RSK (ribosomal S kinase), MAPK (MAP kinase) or GSK-3 (glycogen synthase kinase) leading ultimately to changes in gene expression G protein signal transduction - what is secondary messenger? - ANSWER - cAMP ...

BCH5413 TEST 2 QUESTIONS AND ANSWERS ALL REVISED AND UPDATED Methylation: a primitive immune system for bacteria - hds-R - Answer-- plasmid transforms into cells are protected (restriction enzyme not expressed) Methylation: a primitive immune system for bacteria - mcrA/mcrB/mrr - Answer-degrades foreign DNA that is not properly methylated - can occur to DNA obtained from mouse/human cells containing CpG methylated DNA (can interfere with cloning of mammalian genomic DNA) pCR 2.1 - TOP...

When explaining genetic coding to a group of students, the instructor discusses gene activation and deactivation. It was stressed that inactivation of a gene requires which of the following processes? Correct Answer-Methylation of histone amino acid When an infant is born with gene mutations in his cells, the nurse explains to the parents that accidental errors may be a result of: Select all that apply. Correct Answer-Loss of one or more base pairs Substitution of one base pair for another ...

The Klenow fragment of E. coli DNA polymerase I is used: correct answersto label DNA probes by random primer labeling. What is a solution hybridization method for the detection of nucleic acid:protein interactions? correct answersgel mobility shift assay _____ involves mixing a labeled oligonucleotide with a protein extract to see if a specific interaction occurs, visible with an apparent increase in molecular size on a gel due to the protein:nucleic acid interaction. correct answersGel mo...

Give two examples of how eukaryotic organisms derive benefits from regulating gene expression - 1) Rabbits change from brown to white due to a response to environmental changes 2) larval to adult is totally different b/c of different proteins during life changes gene expression control is used in multicellular organisms to. . . - define tissues and cells w/ specific functions w/in organism What is the order of regulation of gene expression - transcription, RNA processing, translation, post...

Predominant form of DNA -Correct Answer Right-handed B DNA and histone interaction is due to what type of bond? -Correct Answer Ionic What stains to the minor groove of DNA duplex? -Correct Answer SYBR Green What is the most abundant type of RNA? -Correct Answer Ribosomal RNA (rRNA) What does RNA act as in a retrovirus? -Correct Answer Genome and mRNA What increases half-life of mRNA? -Correct Answer 5' Methyl Cap What kind of DNA is most likely to be transcriptionally active?...