Phase contrast microscopy Study guides, Class notes & Summaries

Which of the following family of viruses have DNA genomes Correct Answer-Hepadnaviridae The single stranded negative strand RNA of a virus Correct Answer-must be converted to positive strand RNA in order to be translated into proteins The distinction between a prion and a virus is that Correct Answer-A prion is a single protein molecule whereas a virus may contain many copies of one or several proteins The Pasteur Swan Neck flask experiment shows that Correct Answer-microorganisms are p...

What is different about phase contrast microscopy compared to brightfield? You are able to visualize things that would otherwise be invisible; cells are highly distinguishable True or False: A cell that is adherent, flat (thin), and unstained is easily identified using bright field microscopy False: Adherent, flat cells are almost invisible due to the limits on both resolution and contrast True or False: The distinguishing characteristic of Gram-Negative bacteria is the presence of LPS...

Resolution Correct Answer-small dist b/t 2 pts can be distinguished; D = .61λ/Nsinα, want lower D; Light microscope 200nM Bright field microscopy Correct Answer-normal light type Phase-contrast Correct Answer-altered phase interference of unobstructed light; looks like cells are surrounded by light DIC Correct Answer-3D looking cells Confocal Correct Answer-3D imaging with fluorescence, progessively capture full surface in focus by removing out of focus; normal point-scanning vs....

Light microscopy CORRECT ANSWER Limited ability to visualize bacterial components or distinguish between bacteria without doing something extra Electron microscopy CORRECT ANSWER Can view exterior and internal structures, smallest specimens Phase-contrast microscopy CORRECT ANSWER Refracts the light that goes through the specimen; can see detail, live cells Fluorescence microscopy CORRECT ANSWER Use of fluorochromes that emit light of specific wavelengths when excited by light of a diff...

beta-oxidation correct answer: enzymes repeatedly split off pairs of they hydrogenated carbon atoms that make up a fatty acid and join each pair to coenzyme A to form acetyl-CoA Chloroplasts correct answer: light harvesting structures found in photosynthetic eukaryotes; synthesize a few polypeptides compare and contrast archaeal flagella with bacterial flagella correct answer: archaeal are about half as thick as bacteria; not hollow; lack a central channel; flow together; powered by ATP...

Portage Learning: Microbiology Module 3 Already Graded A Define the measurements micrometer and nanometer. A micrometer (μm) is defined as being one-millionth of a meter and is commonly designated at 10-6 meters. A nanometer (nm) equals 10-9 m or one-billionth of a meter. What are the two critical factors that influence your ability to see an object? Resolution and contrast. Resolution refers to the distance between two objects at which the objects still can be seen as separate. Poor or low r...

Which term is used to refer to a wave's rate of vibration? Ans- B. frequency On the figure, which letter represents a refracted ray of light? Ans- C (BUT D on test) On the electromagnetic spectrum, which type of wave has the shortest wavelength? Ans- A. cosmic ray Dyes are often used during microscopy to increase which of the following? Ans- A. contrast Which of the following was the first to use the term "cells"? Ans- A. Robert Hooke Which of the following is the typical magnific...

BIO 353 test 1 multiple choice -ASU questions & answers 2024 A student in a cellular biology laboratory is faced with the task of detecting protein X in muscle cells taken from a rat. The tissue is required to be live while under analysis. Which type of microscopy should be employed? a) Phase contrast light microscopy b) Transmission electron microscopy c) Scanning electron microscopy d) Fluorescence microscopy e) None of the above - ANSWER-d) Fluorescence microscopy Which...

Select the appropriate type of microscopy to perform for a given specimen and experimental question. CORRECT ANSWER viruses-->nanometers e. coli-->micrometers yeast-->micrometers period (on a page)-->millimeters types of microscopy -light: x1000-x2000 mag can't see in much detail, need to stain to see more -TEM: x500,000 mag (exterior; living) -SEM: x500,000 mag (really small; internal struct; dead) labeling techniques -staining: can clearly see struct, kills the cell -...

Electron beams can not be used to resolve images of objects. T/F? Correct Answer-False In all types of electron microscopy, electrons pass through the specimen. Correct Answer-False This type of light microscopy can be used to view very thin cells or flagella. It does that by using this property of light. Correct Answer-Dark field; Light scattering In this type of microscopy, light is delayed by passing through the sample and so objects look as if they are out of phase. It employs this ...