Western blotting - Study guides, Class notes & Summaries

GCD Quiz 7/8 Practice 69 Questions with Verified Answers Which of the following may be used as a vector in a gene-cloning experiment? a.mRNA b.Plasmid c.Virus d.Both b and c - CORRECT ANSWER d The restriction enzymes used in gene-cloning experiments _______, which generates sticky ends that can _______. the DNA, enter bacterial cells the DNA, hydrogen bond with complementary sticky ends late DNA, enter bacterial cells late DNA, hydrogen bond with complementary sticky ends - CORRE...

OpenStax Microbiology Test Bank Chapter 20: Laboratory Analysis of the Immune Response * = Correct answer Multiple Choice 1. Most antigens have how many epitopes? A. none B. one C. two D. more than two* 2. Which best describes monoclonal antibodies? A. They are produced in living animals only. B. They are produced in tissue cultures, using B cells extracted from living animals.* C. They are rarely used, so polyclonal antibodies are preferred. D. They can be produced in either liv...

Albert's Chapter 8 Exam 115 Questions with Verified Answers WITTF: machine that sorts fluorescently labeled cells and nonlabeled cells into separate containers using a strong electric field - CORRECT ANSWER fluorescence-activated cell sorter How are antibodies used to fluorescently label cells? - CORRECT ANSWER fluorescent molecule attached to antibody, which binds to a very specific cell type How does a fluorescent-activated cell sorter work? - CORRECT ANSWER individual cells travel s...

1 MCAT - BIO/BIOCHEM EXAM | QUESTIONS & ANSWERS (VERIFIED) | LATEST UPDATE | GRADED A+ defunct mRNA translation in bacteria would... Correct Answer: prevent cells from replicating. lysogenic cycle Correct Answer: a viral reproductive cycle in which the viral DNA is added to the host cell's DNA and is copied along with the host cell's DNA Lytic phase or lytic cycle Correct Answer: phage replicates immediately, destroying the host cell (All phages carry out this life cycle) prote...

Native-PAGE - Answer-A polyacrylamide gel electrophoresis method for proteins using NON-DENATURING conditions. Proteins keep their native charge and structure so they are separated based on charge and size. SDS-PAGE - Answer-A polyacrylamide gel electrophoresis method for proteins using DENATURING conditions. Sodium Dodecyl Sulfate denatures the proteins and gives the proteins a uniform charge. This allows them to be separated solely on mass, thus, you can estimate the protein's molecular ma...

OpenStax Microbiology Test Bank Chapter 20: Laboratory Analysis of the Immune Response * = Correct answer Multiple Choice 1. Most antigens have how many epitopes? A. none B. one C. two D. more than two* 2. Which best describes monoclonal antibodies? A. They are produced in living animals only. B. They are produced in tissue cultures, using B cells extracted from living animals.* C. They are rarely used, so polyclonal antibodies are preferred. D. They can be produced in either liv...

MMSC490 Practice Exam 1|123 Questions with Verified Answers cytoplasmic organelles are: a. absent in prokaryotic cells and present in eukaryotic cells. b. present in both prokaryotic and eukaryotic cells. c. present in prokaryotic cells and absent in eukaryotic cells. d. absent in both prokaryotic and eukaryotic cells. - CORRECT ANSWER a. absent in prokaryotic cells and present in eukaryotic cells E. coli is a useful model system for molecular biology studies because: a. it has a smal...

An experiment with bacteria that has been exposed to an antibiotic reveals that the translocation step has been inhibited. Which antibiotic was likely used? correct answers none of these answers are correct. The correct mRNA secondary structure of transcriptional termination is __________ stem-loop structure. correct answers 3-4 In negative autoregulation, correct answers proteins repress their own gene expression. Which sequence is MOST likely to be prenylated in the ER? (The ... repre...

what is the purpose of Polymerase chain reaction (PCR)? correct answer: diagnostic tool to amplify a desired DNA fragment. What do you need for PCR? correct answer: Excess PRIMERS, Excess dNTPs, DNA polymerase (taq) What is the purpose of DNA polymerase ? correct answer: makes polymers of any molecules what is a chain reaction ? correct answer: a reaction that progress in an exponential way 2--4--6--8--10 why making copied of DNA ? correct answer: diagnosing infections, crime ...

OpenStax Microbiology Test Bank Chapter 20: Laboratory Analysis of the Immune Response * = Correct answer Multiple Choice 1. Most antigens have how many epitopes? A. none B. one C. two D. more than two* Difficulty: Easy ASM Standard: N/A 2. Which best describes monoclonal antibodies? A. They are produced in living animals only. B. They are produced in tissue cultures, using B cells extracted from living animals.* C. They are rarely used, so polyclonal antibodies are preferred. ...