Affinity chromatography - Study guides, Class notes & Summaries

Albert's Chapter 8 Exam 115 Questions with Verified Answers WITTF: machine that sorts fluorescently labeled cells and nonlabeled cells into separate containers using a strong electric field - CORRECT ANSWER fluorescence-activated cell sorter How are antibodies used to fluorescently label cells? - CORRECT ANSWER fluorescent molecule attached to antibody, which binds to a very specific cell type How does a fluorescent-activated cell sorter work? - CORRECT ANSWER individual cells travel s...

The tertiary level of 3D protein structure is stabilized by a) ester bonds only b) hydrogen bonds only c) interaction with the molecular environment d) glycosidic bonds e) none of these is correct correct answers c) interaction with the molecular environment Which one of these chemical bonds is planar? a) C-C b) peptide bond c) glycosidic bond d) hydrogen bond e) ionic bond correct answers b) peptide bond in the figure the protein structure depicted by the letter D can be bes...

Thin layer chromatography can use silica plates which are very polar. Will polar or non polar substances have lower Rf values on this plate? ANS -Polar substances - they will have a higher affinity for the silica and thus will not travel as far as the non polar substances A titration of a strong acid with a strong base or vice versa has an equivalence point around what pH? ANS -7 What type of bond is usually disrupted during denaturation of proteins? ANS -H bond Is uracil a purine or...

Thin layer chromatography can use silica plates which are very polar. Will polar or non polar substances have lower Rf values on this plate? ANS -Polar substances - they will have a higher affinity for the silica and thus will not travel as far as the non polar substances A titration of a strong acid with a strong base or vice versa has an equivalence point around what pH? ANS -7 What type of bond is usually disrupted during denaturation of proteins? ANS -H bond Is uracil a purine or...

Clinical Chemistry Exam 2 Questions and Answers 100% Pass The preferred collection tube for preservation of glucose is a) Calcium Oxalate b) Sodium fluoride c) Lithium Heparin d) Sodium Citrate sodium fluoride Glycated hemoglobin is measured in units of: a) Gram % b) Milligram % c) % of total hemoglobin d) % of total protein % of total hemoglobin Electrolyte disturbances seen in Type I diabetic ketoacidosis include: a) Decreased plasma calcium b) Increased plasma potassium c) Increas...

Buffer Order and Henderson Hasselbach - Water, Buffer, Salt, Base pH = pKa + log(A/HA) pKa decrease as temp increase so pH decrease Ramachandran plots - Φ = N-R ψ = C-R optimal angle to prevent steric hinderance Protein structure of a helix, B sheet, reverse turn - a: LEMARKHQ B: VICYFTW RT: PSDNG how many amino acids in alpha turn helix - 3.6 Super secondary structure - helix turn helix parallel vs. antiparallel - parallel wedges face same way antiparallel face opposite ...

carrier gas - correct answers MP gase in GC cold trapping - correct answers Splitless gas chromatography injection technique in which solute is condensed far below its boiling point in a narrow band at the start of the column. electron capture detector - correct answers gas chromatography detector that is particularly sensitive to compounds with halogen atoms, nitro groups, and other groups with high electron affinity flame ionization dectector - correct answers gas chromatography detec...

carrier gas - correct answers mobile phase gas in gas chromatography cold trapping - correct answers splitless gas chromatography injection technique in which solute is condensed far below its boiling point in narrow band at the start of the column electron capture detector - correct answers gas chromatography detector that is particularly sensitive to compounds with halogen atoms, nitro groups, and other groups with high electron affinity flame ionization detector - correct answers A g...

Units 1-5 - Memorize all 20 amino acids - Memorize what all the complex carbs look like and a few facts about them - Know Michaleis-Menton graph and be able to identify type of enzyme inhibitor - Be able to explain how to get Vmax and Km from Lineweaver-Burk - Know how to translate DNA to mRNA and know the peptide that would be translated o do not need to memorize codons - Outline steps of translation - Know about all categories of: o Lipids o Active/passive transport o Plasmids o Re...

ACEDS/ACTUAL EXAM LATEST VERSION WITH VERIFIED QUESTIONS AND ANSWERS GRADED A+ Henderson-Hasselbach Equation - ANS--pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - ANS--Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Collagen - ANS--Repeating sequence of Gly-X-Pro. 3 stranded "coiled coil". Contains gly core. Myoglobin 4° Structure - ANS--Symmetric homodimer, Salting Out (Purification) - ANS--Changes...