Microbiology lab stuvia - Study guides, Class notes & Summaries

Straighterline Microbiology Lab BIO250L Lab 7 Microbial Genetics & Genetic Engineering Worksheet (New Version Updated)

What is the purpose of a streak plate? - ANSWERS1) To separate cells so that they grow to form isolated colonies 2) To confirm if a culture is a pure colony or mixed 3) To confirm is there is contamination in your culture What is a colony? - ANSWERSAreas of growth that arise from a single cell. How do colonies form? - ANSWERSThey are formed by spreading out cells. Cells are spread and reproduce over and over again through bionary fission until visable mass of cells appear. What is a pur...

What three color pencils are needed? - ANSWER - blue, red, green How are endospores freed form the vegetative cell in which they form? - ANSWER - released when losses the surrounding sporangium What drugs are used to treat tuberculosis? - ANSWER - rifampin, isoniazid, ehtambutol, and pyrazinamide What drugs are used to treat leprosy? - ANSWER - rifampin What other disease besides tuberculosis is caused by pathogenic mycobacterium? In what animal to they grow? - ANSWER - leprosy (Hans...

Convert Celsius to Fahrenheit - ANSWER - F = (9xC)/5 +32 = How many decimel places, to the right of base - ANSWER - Six places --- .000 000 nm= How many decimel places, to the right of base - ANSWER - Nine places --- . 6.02x 10 = moving the decimel over how many spaces? - ANSWER - 23 Compare and contrast these three techniques by where bacteria grow in the media - ANSWER - Streak Plate - bacteria grow only on the surface Pour Plate - bacteria grow throughout the media Spread Plate ...

Why is coagulase positive staphylococcus more virulent that then coagulase negative? - ANSWER - Because the clotting factor which is bad.. Positive = MRSA strain which is resistant Are all streptococci Streptococcus sp. ? - ANSWER - No Are all Streptococcus sp. streptococci? - ANSWER - Yes What is a quick test to help distinguish between Streptococcus and Staphylococcus? - ANSWER - Catalase Are all streptococci negative? - ANSWER - Yes Are all staphylococci catalase positive? -...

simple microscope - ANSWER - a microscope that contains only one lens compound microscope - ANSWER - microscope that allows light to pass through a specimen and uses two lenses to form an image field of view - ANSWER - how much of the specimen you can see depth of field - ANSWER - how much of a vertical area remains in focus working distance - ANSWER - how far the objective is from the slide low power objective - ANSWER - provides a magnification of 10x and is the shortest objecti...

Oral consumption (eating, drinking, applying chap stick) is permitted in the microbiology lab - ANSWERSunder no circumstances. When is it okay to touch one's face/eyes/mouth/nose in the microbiology lab? - ANSWERSIt's never okay. What is the very first thing you should do when you enter the lab? - ANSWERSDisinfect your bench. What is the very last thing you should do before leaving the microbiology lab? - ANSWERSWash your hands. When working with hazardous chemicals and/or live mic...

Klebsiella pneumoniae - ANSWERSRod-shaped Has capsule Corynebacterium xerosis - ANSWERSRod-shaped no capsule Enterobacter aerogenes - ANSWERSRod-shaped Bacillus megaterium/subtillis - ANSWERSRod-shaped Gram-positive Strict aerobe Endospore-forming Escherica coli - ANSWERSRod-shaped Gram-negative Growth and ferment in MSA and MAC Gamma hemolytic Facultative anaerobe Motile Staphylococcus epidermidis - ANSWERSCocci-shaped Gram-positive Grow in MSA Gamma hemolytic Not m...

What is the purpose of a streak plate? - ANSWERS1) To separate cells so that they grow to form isolated colonies 2) To confirm if a culture is a pure colony or mixed 3) To confirm is there is contamination in your culture What is a colony? - ANSWERSAreas of growth that arise from a single cell. How do colonies form? - ANSWERSThey are formed by spreading out cells. Cells are spread and reproduce over and over again through bionary fission until visable mass of cells appear. What is a pur...

Oral consumption (eating, drinking, applying chap stick) is permitted in the microbiology lab - ANSWERSunder no circumstances. When is it okay to touch one's face/eyes/mouth/nose in the microbiology lab? - ANSWERSIt's never okay. What is the very first thing you should do when you enter the lab? - ANSWERSDisinfect your bench. What is the very last thing you should do before leaving the microbiology lab? - ANSWERSWash your hands. When working with hazardous chemicals and/or live mic...