Southern blotting - Study guides, Class notes & Summaries

MTM Exam 4 Diseases Latest Update with Verified Solutions DMD -loss of function, recessive, haplosufficiency -symptoms present < 5 yoa -progressive symmetrical muscular weakness -wheelchair dependent by 12-13 yoa -life expectancy = teens - 30s -do not reproduce -calf pseudohypertrophy -elevated serum creatine kinase -unable to perform neck flexion -complete absence of dystrophin -mutations: deletions, frameshift, nonsense -exon length is NOT a multiple of 3 -frameshifts -> null mutations ...

FRNSC 421W - EXAM 1 PRACTICE QUESTIONS AND ANSWERS (100% PASS) RFLP - Answer️️ -restriction fragment length polymorphism - DNA digested by restriction enzymes at repeat sequences, yields different fragment lengths for different people - Gel electrophoresis to separate fragments by size, MW ladder for reference - Southern blotting to physically transfer DNA to membrane - Radioactively labeled probe hybridizes with immobilized DNA on membrane - Exposure to X-ray film yields carbon copy...

1 MCAT - BIO/BIOCHEM EXAM | QUESTIONS & ANSWERS (VERIFIED) | LATEST UPDATE | GRADED A+ defunct mRNA translation in bacteria would... Correct Answer: prevent cells from replicating. lysogenic cycle Correct Answer: a viral reproductive cycle in which the viral DNA is added to the host cell's DNA and is copied along with the host cell's DNA Lytic phase or lytic cycle Correct Answer: phage replicates immediately, destroying the host cell (All phages carry out this life cycle) prote...

Native-PAGE - Answer-A polyacrylamide gel electrophoresis method for proteins using NON-DENATURING conditions. Proteins keep their native charge and structure so they are separated based on charge and size. SDS-PAGE - Answer-A polyacrylamide gel electrophoresis method for proteins using DENATURING conditions. Sodium Dodecyl Sulfate denatures the proteins and gives the proteins a uniform charge. This allows them to be separated solely on mass, thus, you can estimate the protein's molecular ma...

MMSC490 Practice Exam 1|123 Questions with Verified Answers cytoplasmic organelles are: a. absent in prokaryotic cells and present in eukaryotic cells. b. present in both prokaryotic and eukaryotic cells. c. present in prokaryotic cells and absent in eukaryotic cells. d. absent in both prokaryotic and eukaryotic cells. - CORRECT ANSWER a. absent in prokaryotic cells and present in eukaryotic cells E. coli is a useful model system for molecular biology studies because: a. it has a smal...

Define biotechnology - Answer- The use of technology to improve living organism and processes for the bennifit of man Briefly state the main difference of modern from classical biotechnology - Answer- Modern BTEC involves recombinant DNA technology Selective breeding is an early form of classical biotech, where there is deliberate crossing of parent organisms with favorable traits. Name two vegetables that are products of selective breeding from one common ancestor that were shown in the l...

QBM Exam 3 Questions And Answers With Complete Solutions 2024 Which of the following DNA purification techniques CANNOT be used for genomic DNA purification? - answerminiprep Which DNA purification technique involves purifying the sample using an ultracentrifuge? - answerCesium chloride gradient What technique is used to increase plasmid copy number and purify plasmids? - answerMiniprep. Which of the following wavelengths is incorrectly matched? - answer240 nm - RNA concentration. The ...

BIO 1232 - SEMI FINAL PART A AND B QUESTIONS AND ANSWERS 100% CORRECT. Cell Biology 1. Which of the following is the most important type of force used to stabilize secondary structure in proteins? a) Ionic bonds b) Hydrogen bonds c) Aromatic-aromatic ring interactions d) Hydrophobic Interactions e) Peptide bonds 2. Which of the following is NOT true of allosterically regulated enzymes? a) they follow Michaelis-Menten kinetics b) they demonstrate cooperativity c) their activity can b...

QBM Exam 3 Questions And Answers With Complete Solutions 2024 Which of the following DNA purification techniques CANNOT be used for genomic DNA purification? - answerminiprep Which DNA purification technique involves purifying the sample using an ultracentrifuge? - answerCesium chloride gradient What technique is used to increase plasmid copy number and purify plasmids? - answerMiniprep. Which of the following wavelengths is incorrectly matched? - answer240 nm - RNA concentration. The ...

MB ASCP Test Molecular Techniques (20-30%) Questions and Answers All correct What are the 3 types of gel electrophoresis Agarose Polyacrylamide Pulse Field What is gel electrophoresis the separation of materials based on size and migration patterns when subjected to electrical forces in a resistive medium. which direction does the DNA flow in gel electrophoresis due to DNA being negatively charged, it will migrate towards the positive end What determines how far the DN...