Western blotting - Study guides, Class notes & Summaries

Buffer Order and Henderson Hasselbach - Water, Buffer, Salt, Base pH = pKa + log(A/HA) pKa decrease as temp increase so pH decrease Ramachandran plots - Φ = N-R ψ = C-R optimal angle to prevent steric hinderance Protein structure of a helix, B sheet, reverse turn - a: LEMARKHQ B: VICYFTW RT: PSDNG how many amino acids in alpha turn helix - 3.6 Super secondary structure - helix turn helix parallel vs. antiparallel - parallel wedges face same way antiparallel face opposit...

ACS Biochemistry First Semester Exam Questions with All Correct Answers Buffer Order and Henderson Hasselbach - ANSWER Water, Buffer, Salt, Base pH = pKa + log(A/HA) pKa decrease as temp increase so pH decrease Ramachandran plots - ANSWER Φ = N-R ψ = C-R optimal angle to prevent steric hinderance Protein structure of a helix, B sheet, reverse turn - ANSWER a: LEMARKHQ B: VICYFTW RT: PSDNG how many amino acids in alpha turn helix - ANSWER 3.6 Supe...

Define biotechnology - Answer- The use of technology to improve living organism and processes for the bennifit of man Briefly state the main difference of modern from classical biotechnology - Answer- Modern BTEC involves recombinant DNA technology Selective breeding is an early form of classical biotech, where there is deliberate crossing of parent organisms with favorable traits. Name two vegetables that are products of selective breeding from one common ancestor that were shown in the l...

AAMC full length 2, EXAM Overviews 2023 Update amide linking is peptide on the carboxyl side - ANS-so amide linkage involving the N atom in AMC used to covalently attach flurophore to the peptide what expression gives the amount of light energy (J/photon) that is converted to other forms between the fluorescence excitation and emission events? - ANS-E=hf = hc/lambda, here we see that excitation is at 360 nm but observation is at 440 nm. so you have E = (6.62 x 10^-34)(3x 10^8)x (1/360...

MB ASCP Test Molecular Techniques (20-30%) Questions and Answers All correct What are the 3 types of gel electrophoresis Agarose Polyacrylamide Pulse Field What is gel electrophoresis the separation of materials based on size and migration patterns when subjected to electrical forces in a resistive medium. which direction does the DNA flow in gel electrophoresis due to DNA being negatively charged, it will migrate towards the positive end What determines how far the DN...

Intact growth factors are conserved in the extracellular matrix of ancient human bone and teeth: a storehouse for the study of human evolution in health and disease Tyede H. Schmidt-Schultz1 and Michael Schultz2, * 1Department of Biochemistry, University of Go¨ ttingen, D-37075 Go¨ ttingen, Germany 2Department of Anatomy, University of Go¨ ttingen, D-37075 Go¨ ttingen, Germany * Corresponding author e-mail: Abstract For the first time we have extracted, solubilized and identif...

AP Bio Genetics, RNA, and DNA Practice Exam 110 Questions with Verified Answers In his transformation experiments, what did Griffith observe? a. Mutant mice were resistant to bacterial infections. b. Mixing a heat-killed pathogenic strain of bacteria with a living nonpathogenic strain can convert some of the living cells into the pathogenic form. c. Mixing a heat-killed nonpathogenic strain of bacteria with a living pathogenic strain makes the pathogenic strain nonpathogenic. d. Infecti...

What are the 3 types of gel electrophoresis Agarose Polyacrylamide Pulse Field What is gel electrophoresis the separation of materials based on size and migration patterns when subjected to electrical forces in a resistive medium. which direction does the DNA flow in gel electrophoresis due to DNA being negatively charged, it will migrate towards the positive end What determines how far the DNA will travel in a gel the size of the DNA fragment What factors affec...

Laboratories that work with moderate risk pathogens are classified as BSL-2 Bacteremia is a term used to indicate the presence of bacteria in the blood. 00:29 01:22 Which of the following must be accomplished FIRST in order to conduct RT-PCR? Complementary DNA must be synthesized from RNA The most common of all nosocomial infections are ________ infections urinary tract MacConkey agar and eosin-methylene blue (EMB) agar are ________ media. both selectiv...

OpenStax Microbiology Test Bank Chapter 20: Laboratory Analysis of the Immune Response * = Correct answer Multiple Choice 1. Most antigens have how many epitopes? A. none B. one C. two D. more than two* 2. Which best describes monoclonal antibodies? A. They are produced in living animals only. B. They are produced in tissue cultures, using B cells extracted from living animals.* C. They are rarely used, so polyclonal antibodies are preferred. D. They can be produced in either liv...