Ascp mb ii - Study guides, Class notes & Summaries

MB (ASCP) Review & Exam Questions Part II Already Graded A+ (2023) What is the Beer-Lambert Law? - Answer the linear relationship between absorbance and concentration of an absorbing species. What is the Beer-Lambert Law equation? - Answer A = ebc A: absorbance e: molar absorptivity c: concentration b: path length DNA and RNA are absorbed at: - Answer 260nm Proteins are absorbed at: - Answer 280nm What is the melting temperature equation? - Answer Tm = (4 x # of GC pairs) + (2 x # of ...

Technologist in Molecular Biology, MB(ASCP) board exam prep 2024 with 100% Correct Answers Pyrimidine - Answer ️️ -One carbon ring Cytosine, Thymine, Uracil What is the function of mRNA? - Answer ️️ -Carries genetic info out of nucleus Transcript translated to protein What is the function of tRNA? - Answer ️️ -Carries aa to ribosome Anticodon pairs with codon on mRNA strand What is the function of rRNA? - Answer ️️ -part of ribosome structure most abundant RNA coordina...

Technologist in Molecular Biology, MB(ASCP) board exam prep 2024 with 100% Correct Answers Pyrimidine - -One carbon ring Cytosine, Thymine, Uracil Purine - -Two carbon rings Adenine, Guanine How are nucleotides joined together? - -Condensation to form phosphodiester bond What is the function of mRNA? - -Carries genetic info out of nucleus Transcript translated to protein What is the function of tRNA? - -Carries aa to ribosome Anticodon pairs with codon on mRNA strand What is the func...

MB ASCP Review and Exam Questions Part II (Already Graded A+ 2023) This analytical test amplifies the same target in two PCR reactions and uses the product of round 1 as the template for round 2. So there are 2 pairs of primers and 2 successive reactions. Basically you pre-amplify a broad region and then amplify a specific region. This test is good to use if you have low amounts of target because it increases specificity/sensitivity. - Answer Nested PCR This analytical test has multiple pai...

ASCP MB: Extra-Practice Questions and Answers Scored A The Klenow fragment of E. coli DNA polymerase I is used: - Answer ️️ -to label DNA probes by random primer labeling. What is a solution hybridization method for the detection of nucleic acid:protein interactions? - Answer ️️ -gel mobility shift assay _____ involves mixing a labeled oligonucleotide with a protein extract to see if a specific interaction occurs, visible with an apparent increase in molecular size on a gel due to...

MB (ASCP) Exam TEST VERIFIED SOLUTIONS Predominant form of DNA - ANSWER Right-handed B DNA and histone interaction is due to what type of bond? - ANSWER Ionic What stains to the minor groove of DNA duplex? - ANSWER SYBR Green What is the most abundant type of RNA? - ANSWER Ribosomal RNA (rRNA) What does RNA act as in a retrovirus? - ANSWER Genome and mRNA What increases half-life of mRNA? - ANSWER 5' Methyl Cap What kind of DNA is most likely to be transcriptionally active? - ANSWER E...

purple / lavender - ANSWER - tripotassium EDTA (7.5-15% solution) - virology, molecular biology studies light blue - ANSWER - Tri-Sodium Citrate - Prothrombin time (PT), Activated Partial Thromboplastin Time (APTT), Fibrinogen thrombin time and other blood Coagulation tests. green top collection tube - ANSWER - sodium heparin (freeze-dried) - immunology, virology studies yellow top collection tube - ANSWER - Acid citrate dextrose (ACD) - molecular biology studies. Blood Bank studies, HL...

MB (ASCP) 100% VERIFIED SOLUTIONS LATEST UPDATES what does topoisomerase do? - ANSWERbreaks phosphate backbone to "un" supercoil DNA Another name for Topoisomerase II - ANSWERGyrase Helicase - ANSWERUn"zips" DNA at replication fork What makes up the primosome? - ANSWER1. primase 2. helicase 3. DNA C NASBA stands for... - ANSWERnucleic acid sequence based amplification NASBA - ANSWERRNA is target RNA-->cDNA Reverse transcriptase (RNA-->cDNA) followed by DNA dependent ...

MB (ASCP) Exam Questions and Answers Graded A Predominant form of DNA - Answer ️️ -Right-handed B DNA and histone interaction is due to what type of bond? - Answer ️️ -Ionic What stains to the minor groove of DNA duplex? - Answer ️️ -SYBR Green What is the most abundant type of RNA? - Answer ️️ -Ribosomal RNA (rRNA) What does RNA act as in a retrovirus? - Answer ️️ -Genome and mRNA What increases half-life of mRNA? - Answer ️️ -5' Methyl Cap What kind of DNA is mo...

MB (ASCP) Exam TEST VERIFIED SOLUTIONS Predominant form of DNA - ANSWER Right-handed B DNA and histone interaction is due to what type of bond? - ANSWER Ionic What stains to the minor groove of DNA duplex? - ANSWER SYBR Green What is the most abundant type of RNA? - ANSWER Ribosomal RNA (rRNA) What does RNA act as in a retrovirus? - ANSWER Genome and mRNA What increases half-life of mRNA? - ANSWER 5' Methyl Cap What kind of DNA is most likely to be transcriptionally active? - ANSWER E...