Dna extraction - Study guides, Class notes & Summaries

MB (ASCP) 100% VERIFIED SOLUTIONS LATEST UPDATES what does topoisomerase do? - ANSWERbreaks phosphate backbone to "un" supercoil DNA Another name for Topoisomerase II - ANSWERGyrase Helicase - ANSWERUn"zips" DNA at replication fork What makes up the primosome? - ANSWER1. primase 2. helicase 3. DNA C NASBA stands for... - ANSWERnucleic acid sequence based amplification NASBA - ANSWERRNA is target RNA-->cDNA Reverse transcriptase (RNA-->cDNA) followed by DNA dependent ...

Molecular Diagnostics Chapter 3 Exam 100% Solved Which sample types must be amplified prior to analysis? - answerbuccal cells, saliva, bone, teeth, hair, dried blood, fixed tissue What are two ways to break down cell walls for DNA extraction? - answer1. homogenization through physical grinding 2. treatment of detergent (SDS) with strong base (NaOH) in ethylene- diaminetetraacetic acid (EDTA) Ficoll - answerHighly branched sugar Solution w/ specific density that allows only RBCs and neut...

Test Bank for Hole’s Human Anatomy and Physiology, 16th Edition, Charles Welsh, Cynthia Prentice-Craver, ISBN10: 6 by Charles Welsh, Cynthia Prentice-Crave. CHAPTER 1 TRUE/FALSE - Write 'T' if the statement is true and 'F' if the statement is false. 1) Paired organs that are bilateral, on the left and right side of the body, would be separated by a midsagittal section. ⊚ true ⊚ false 2) The transition from a hunter-gatherer to an agricultural lifestyle greatly changed the t...

Concerning antigen-antibody reactions, the prozone effect causes which of the following results? A. False positive B. False negative C. No reaction D. Mixed field reaction B. False negative The following characteristics are applicable to Interferon-Gamma Release Assays (IGRAs) except: A. Can be used in patients with active tuberculosis disease symptoms B. Preferable for patients who have received a bacillus Calmette-Guérin (BCG) vaccination C. Can be used instead of purified ...

Molecular diagnostics: fundamentals methods and clinical applications: ch 4 Molecules that make up DNA backbone - ANS-Ribose Sugar and phosphate nitrogen base bound to an unphosphorylated sugar - ANS-nucleoside starting source of organic and inorganic DNA isolation - ANS-cells in suspension Four general steps of organic DNA isolation - ANS-lysis, acidification, phenol chloroform extraction, DNA precipitation Three general steps for inorganic DNA isolation - ANS...

Concerning antigen-antibody reactions, the prozone effect causes which of the following results? A. False positive B. False negative C. No reaction D. Mixed field reaction B. False negative The following characteristics are applicable to Interferon-Gamma Release Assays (IGRAs) except: A. Can be used in patients with active tuberculosis disease symptoms B. Preferable for patients who have received a bacillus Calmette-Guérin (BCG) vaccination C. Can be used instead of purified ...

QBM Exam 3 Questions And Answers With Complete Solutions 2024 Which of the following DNA purification techniques CANNOT be used for genomic DNA purification? - answerminiprep Which DNA purification technique involves purifying the sample using an ultracentrifuge? - answerCesium chloride gradient What technique is used to increase plasmid copy number and purify plasmids? - answerMiniprep. Which of the following wavelengths is incorrectly matched? - answer240 nm - RNA concentration. The ...

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Molecular Diagnostics Chapter 3 Exam 100% Solved Which sample types must be amplified prior to analysis? - answerbuccal cells, saliva, bone, teeth, hair, dried blood, fixed tissue What are two ways to break down cell walls for DNA extraction? - answer1. homogenization through physical grinding 2. treatment of detergent (SDS) with strong base (NaOH) in ethylene- diaminetetraacetic acid (EDTA) Ficoll - answerHighly branched sugar Solution w/ specific density that allows only RBCs and neut...

QBM Exam 3 Questions And Answers With Complete Solutions 2024 Which of the following DNA purification techniques CANNOT be used for genomic DNA purification? - answerminiprep Which DNA purification technique involves purifying the sample using an ultracentrifuge? - answerCesium chloride gradient What technique is used to increase plasmid copy number and purify plasmids? - answerMiniprep. Which of the following wavelengths is incorrectly matched? - answer240 nm - RNA concentration. The ...