In real time pcr - Study guides, Class notes & Summaries
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3.0 BACE EXAM QUESTIONS AND ANSWERS
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The invention of the thermal cycler 
The use of reverse transcriptase from single-stranded RNA 
The use of fluorescently-tagged probes for real-time quantitation of PCR products - Answer- SIgnificant Advances in PCR include 
 
the structure of DNA was described, as well as its method of replication - Answer- What happened in the 1950s? 
 
the isolation of restriction enzymes - Answer- The advent of recombinant DNA technology began with what? 
 
it allowed us to manipulate DNA - Answer- What did...
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Molecular Dipstick Test for Diagnosis of Sleeping Sickness
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Molecular Dipstick Test for Diagnosis of Sleeping Sickness 
 S. Deborggraeve,1,3* F. Claes,1 T. Laurent,1 P. Mertens,2 T. Leclipteux,2 
 J. C. Dujardin,1 P. Herdewijn,3 and P. Bu ¨scher1 
 Department of Parasitology, Institute of Tropical Medicine, Antwerp, Belgium,1 Coris BioConcept, Gembloux, 
 Belgium,2 and Laboratory of Medicinal Chemistry, Rega Institute for Medicinal Research, Leuven, Belgium3 
 Received 14 December 2005/Returned for modification 27 January 2006/Accepted 2 June 2006 
 Hum...
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Bio 250 Midterm Questions with Correct Answers
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Immunochemistry Correct Answer -used to find antigens, proteins, and cancer in specific tissues via antibodies 
 
Induced pluripotent stem-cell programming Correct Answer -tweaks adult cells into stem cells to force them to express genes, to have properties of embryonic stem cells, to model disease and study drug development 
 
RT-qpcr Correct Answer -used to analyze gene expression (how much is gene expressed?) 
 
Real time PCR Correct Answer -measures telomere length in humans 
 
Western blot ...
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(latest update 2023) MB (ASCP) Review & Exam Questions Part II Already Graded A+
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What is the Beer-Lambert Law? - Answer the linear relationship between absorbance and concentration of an absorbing species. 
 
What is the Beer-Lambert Law equation? - Answer A = ebc 
 
A: absorbance 
e: molar absorptivity 
c: concentration 
b: path length 
 
DNA and RNA are absorbed at: - Answer 260nm 
 
Proteins are absorbed at: - Answer 280nm 
 
What is the melting temperature equation? - Answer Tm = (4 x # of GC pairs) + (2 x # of AT pairs) 
 
Dilutions equation - Answer V1C1 = V2C2 
 
V1:...
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AAB MT IMMUNOLOGY REVIEWED TEST 2023/2024
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AAB MT IMMUNOLOGY REVIEWED 
TEST 2023/2024 
Concerning antigen-antibody reactions, the prozone effect causes which of the following 
results? 
A. False positive 
B. False negative 
C. No reaction 
D. Mixed field reaction - CORRECT ANSWER-B. False negative 
The following characteristics are applicable to Interferon-Gamma Release Assays 
(IGRAs) except: 
A. Can be used in patients with active tuberculosis disease symptoms 
B. Preferable for patients who have received a bacillus Calmette-Guérin...
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GCD Quiz 7/8 Practice 69 Questions with Verified Answers,100% CORRECT
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GCD Quiz 7/8 Practice 69 Questions with Verified Answers 
 
Which of the following may be used as a vector in a gene-cloning experiment? 
a.mRNA 
b.Plasmid 
c.Virus 
d.Both b and c - CORRECT ANSWER d 
 
The restriction enzymes used in gene-cloning experiments _______, which generates sticky ends that can _______. 
 the DNA, enter bacterial cells 
 the DNA, hydrogen bond with complementary sticky ends 
late DNA, enter bacterial cells 
late DNA, hydrogen bond with complementary sticky ends - CORRE...
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AAB MT IMMUNOLOGY REVIEWED TEST 2023/2024
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AAB MT IMMUNOLOGY REVIEWED 
TEST 2023/2024 
Concerning antigen-antibody reactions, the prozone effect causes which of the following 
results? 
A. False positive 
B. False negative 
C. No reaction 
D. Mixed field reaction - CORRECT ANSWER-B. False negative 
The following characteristics are applicable to Interferon-Gamma Release Assays 
(IGRAs) except: 
A. Can be used in patients with active tuberculosis disease symptoms 
B. Preferable for patients who have received a bacillus Calmette-Guérin...
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ASCP MB 2023/2024 ALREADY CORRECTLY ANSWERED -GRADED A+
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ASCP MB 2023/2024 
 
 
E. coli RNA Polymerase II - Answer DNA template, product: mRNA 
 
RNA Polymerase I - Answer DNA template 
Location: Nucleous 
Products: 18s, 5.8s, 28s rRNA 
 
RNA Polymerase II - Answer DNA template 
Location: Nucleus 
Products: mRNA, snRNA 
 
RNA Polymerase III - Answer DNA template 
Location: Nucleus 
Product: tRNA, 5s rRNA, snRNA 
 
Mitochondrial RNA Polymerase - Answer DNA template, product: mRNA 
 
Mammalian DNA polymerase α - Answer DNA template, product: primers 
...
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QBM Exam 1 Questions And Answers With Complete Solutions 2024
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QBM Exam 1 Questions And Answers With 
Complete Solutions 2024 
Chapter 1: Overview of the Molecular Biology 
Geber 1800s: He developed ____ that make ways for molecular biology. 
From 1700s - 1900s there are huge advancements in ___ 
(start studying small molecules) 
1930s- Understanding of ________ happen 
1938s - "Molecular Biology" was written by ______. 
(First time the word molecular biology actually happen) 
1944s - _____ shows DNA was the basis for genes 
1953 - ____ and ____ determine...
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Edexcel A-level biology 2023 with complete solutions questions and answers
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Describe the events of fertilisation that occur after the acrosome reaction. 
-Membrane of sperm and egg cell fuse together 
-cortical granules release contents into zona pellucida 
-Zona pellucida thickens 
-Haploid nuclei fuse 
 
 
 
Explain how phospholipids form a cell surface membrane 
-Hydrophilic parts associate with water 
-Hydrophobic parts repel water 
-a bilayer forms with hydrophobic parts pointing in towards the centre of the bilayer 
 
 
 
Describe the role of the CFTR protein in e...
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