Steps of pcr Study guides, Class notes & Summaries

What is the goal of PCR? Correct Answer-To copy and amplify a DNA sequence. What are the requirements of PCR? Correct Answer-Template, primers, deoxyribosenucelotides, DNA polymerase, buffer What is are the general steps of PCR? Correct Answer- What is the purpose of Microarray/chip based hybridization? How do we accomplish this? Correct Answer-We want to get a GLOBAL profile of gene expression. We do this by determining the number of genes expressed in a cell and with how much abundanc...

WGU Biochemistry OA Review Questions And Answers 2022. The polymerase chain reaction is a tool used to study protein structure. True False Ans - False Feedback PCR is a tool used to amplify a specific segment of DNA. What color is the primer in the following diagram? Red Purple Blue Ans - Red Feedback Recall from the section on DNA replication that DNA polymerase needs a primer to begin DNA synthesis. This requirement means the primers will direct the DNA polymerase to only synthesi...

coding region - comprised of genes that encode proteins (instructions that determine traits) and makes up 1-2% of human DNA. non-coding gene - makes up 98-99% of human DNA, contains STRs and is responsible for regulatory functions such as gene transcription short tandem repeats (STRs) - unique repeating patterns of the same nucleotide sequence. They can be used to differentiate one person from another. DNA Profile - specific pattern of DNA attributes that is obtained in the lab and can b...

Males and Females are equally affected - Autosomal The process of copying DNA in the lab. - Polymerase Chain Reaction (PCR) Template DNA, Nucleotides (dNTPS), DNA Polymerase, and DNA Primers. - What is needed for Polymerase Chain Reaction? Denaturing, Annealing, Elongating - What are the three steps of a Polymerase Chain Reaction (PCR)? Denaturing (DNA is heated up to separate it) - What is the first step of a Polymerase Chain Reaction? Cooling the Polymerase Chain Reaction. Primers stick ...

Concerning antigen-antibody reactions, the prozone effect causes which of the following results? A. False positive B. False negative C. No reaction D. Mixed field reaction - correct answer B. False negative The following characteristics are applicable to Interferon-Gamma Release Assays (IGRAs) except: A. Can be used in patients with active tuberculosis disease symptoms B. Preferable for patients who have received a bacillus Calmette-Guérin (BCG) vaccination C. Can be used instea...

Quiz 2 Biochemistry Lab Chem 378 Name:____________________ 1. Below are restriction enzyme maps of two plasmids. Draw or described in numbers the expected bands of digestion in 1% agarose gel electrophoresis, relative to the 2 bands identified for you as control. 2. Explain the different steps that are involved in a PCR experiment (include all the reagents used in the PCR mixture). 5.5 kb BamHI 3730 PstI 2650 BamHI 3050 PstI 1720 PstI BamH1 PstI BamHI pCAG-EYFP 4000 bp 3000 ...

Bio 202 EXAM 3 QUESTIONS AND CORRECT DETAILED ANSWERS How is recombinant DNA technology used to clone the human GH gene? 1st step! - ANSWER 1. use PCR to obtain the gene (Simply cutting the DNA with restriction enzymes would not be an effective way to isolate a specific gene. The genome would be cut into so many fragments, if you ran a gel it would be a smear! Which fragment is yours? You would need hybridization to isolate your fragment. Wouldn't have much DNA- not like the amo...

After experiencing extreme fatigue and polyuria, a patient's basic metabolic panel is analyzed in the laboratory. The result of the glucose is too high for the instrument to read. The laboratorian performs a dilution using 0.25 mL of patient sample to 750 microliters of diluent. The result now reads 325 mg/dL. How should the technologist report this patient's glucose result? A. 325 mg/dL B. 1300 mg/dL C. 975 mg/dL D. 1625 mg/dL - B; The correct answer for this question is 1300 mg/dL. The ...

Males and Females are equally affected - -Autosomal The process of copying DNA in the lab. - -Polymerase Chain Reaction (PCR) Template DNA, Nucleotides (dNTPS), DNA Polymerase, and DNA Primers. - -What is needed for Polymerase Chain Reaction? Denaturing, Annealing, Elongating - -What are the three steps of a Polymerase Chain Reaction (PCR)? Denaturing (DNA is heated up to separate it) - -What is the first step of a Polymerase Chain Reaction? Cooling the Polymerase Chain Reaction. Primers s...

How does BPA cause cancer? - it disrupts meiosis and mitosis causing breast and prostate cancer. 3 and 7 plastics may contain BPA What is a genetic fingerprint? how do you make one? - -Genetic Fingerprint: DNA uses the differences in base sequences to identify individuals. -DNA fingerprinting involves 3 tools: 1. PCR to get large amounts of DNA 2. Restriction digest with blunt ends to fragment the DNA with precision 3. Gel electrophoresis to separate and visualize the DNA fragments ...