What is denaturation - Study guides, Class notes & Summaries

How does information flow? From DNA to RNA to protein What information is central to modern biotechnology? The use of methods to study and manipulate information about the central dogma. What is the exception to the central dogma? The transcription of RNA into DNA using reverse transcriptase. How does the cycle of the central dogma work? Instructions of DNA are transcribed onto messenger RNA. The ribosomes are able to read the genetic information inscribed on a strange of the ...

Differentiate between complete and incomplete proteins and provide typical sources of each. Complete proteins: Have all nine essential amino acids. Ex. Animal sources of protein (milk, meat) Incomplete proteins: Lack one or more of the essential amino acids. Ex. Plant sources of proteins (legumes) Describe the molecular structure of proteins and the four levels of protein structure. Which bonds are essential to each level of organization? Proteins are composed of long chains of amino acid...

PCR allows for: - amplification of minute amounts of template DNA can generate thousands or millions of copies of that sequence What is one of the most important parameters for a successful PCR? - well designed primers Name 3 variables that influence the success of a PCR reaction - 1. quality of template DNA 2. cycling conditions 3. specificity and stability of PCR primers The target sequence determines what criteria in primer design for PCR? - 1. primer length 2. % GC content 3. m...

The correct answer for this question is 1300 mg/dL. The laboratorian per- formed a 1:4 dilution by adding 0.25 mL (or 250 microliters) of patient sample to 750 microliters of diluent. This creates a total volume of 1000 microliters. So, the patient sample is 250 microliters of the 1000 microliter mixed sample, or a ratio of 1:4. Therefore, the result given by the chemistry analyzer must be multiplied by a dilution factor of 4. 325 mg/dL x 4 = 1300 mg/dL.: After experiencing extreme fatigue and ...

What are Mendel's conclusion? correct answers 1. Characteristics are governed by genes 2. Gametes have one allele per trait 3. Part of alleles segregated in gamete formation 4. Alleles controlling different traits segregate independently of another What is an allele correct answers an alternative version of a gene what makes somatic cells correct answers Male and female gametes Dominant alleles, recessive alleles correct answers Dominant allele masks recessive and expresses Disc...

B; The correct answer for this question is 1300 mg/dL. The laboratorian performed a 1:4 dilution by adding 0.25 mL (or 250 microliters) of patient sample to 750 microliters of diluent. This creates a total volume of 1000 microliters. So, the patient sample is 250 microliters of the 1000 microliter mixed sample, or a ratio of 1:4. Therefore, the result given by the chemistry analyzer must be multiplied by a dilution factor of 4. 325 mg/dL x 4 = 1300 mg/dL. - After experiencing extreme fatigue an...

Thin layer chromatography can use silica plates which are very polar. Will polar or non polar substances have lower Rf values on this plate? ANS -Polar substances - they will have a higher affinity for the silica and thus will not travel as far as the non polar substances A titration of a strong acid with a strong base or vice versa has an equivalence point around what pH? ANS -7 What type of bond is usually disrupted during denaturation of proteins? ANS -H bond Is uracil a purine or...

coding region - comprised of genes that encode proteins (instructions that determine traits) and makes up 1-2% of human DNA. non-coding gene - makes up 98-99% of human DNA, contains STRs and is responsible for regulatory functions such as gene transcription short tandem repeats (STRs) - unique repeating patterns of the same nucleotide sequence. They can be used to differentiate one person from another. DNA Profile - specific pattern of DNA attributes that is obtained in the lab and can b...

Thin layer chromatography can use silica plates which are very polar. Will polar or non polar substances have lower Rf values on this plate? ANS -Polar substances - they will have a higher affinity for the silica and thus will not travel as far as the non polar substances A titration of a strong acid with a strong base or vice versa has an equivalence point around what pH? ANS -7 What type of bond is usually disrupted during denaturation of proteins? ANS -H bond Is uracil a purine or...

WGU c785 GRADED A [LATEST 2023/2024] polymerase chain reaction (PCR) - CORRECT ANSWER process to copy DNA What is needed to complete PCR - CORRECT ANSWER Template DNA, Nucleotides (dNTPS), DNA Polymerase, and DNA Steps of PCR - CORRECT ANSWER denaturation, annealing, elongation denaturation - CORRECT ANSWER loss of normal shape of a protein due to heat or other factor Annealing - CORRECT ANSWER cooled to 50c primers stick and want to copy and add DNA polymerase Elongation - CORRECT...