Bch 3025 Study guides, Class notes & Summaries

A common cloning strategy for introducing foreign genes into plants with Agrobacterium employs all the following features except: A) a selectable antibiotic marker such as kanamycin resistance. B) a shuttle vector with 25 bp T-DNA repeats flanking the foreign gene of choice. C) a Ti plasmid lacking its T-DNA segment. D) active vir gene products from the altered Ti plasmid. E) an ability to induce crown gall formation in infected leaves. - Answer-B) a shuttle vector with 25 bp TDNA repeats ...

2,4-Dinitrophenol and oligomycin inhibit mitochondrial oxidative phosphorylation. 2,4-Dinitrophenol is an uncoupling agent; oligomycin blocks the ATP synthesis reaction itself. Therefore, 2,4-dinitrophenol will: A) allow electron transfer in the presence of oligomycin. B) allow oxidative phosphorylation in the presence of oligomycin. C) block electron transfer in the presence of oligomycin. D) diminish O2 consumption in the presence of oligomycin E) do none of the above. - Answer-A) allow...

Which of the following is not required in the synthesis of fatty acids? A) Acetyl-CoA B) Biotin C) HCO3- (CO2) D) Malonyl-CoA E) NADH - Answer-E) NADH Which of the following is not true of the reaction producing malonyl-CoA during fatty acid synthesis? A) It is stimulated by citrate. B) It requires acyl carrier protein (ACP). C) It requires CO2 (or bicarbonate). D) One mole of ATP is converted to ADP + Pi for each malonyl-CoA synthesized. E) The cofactor is biotin. - Answer-B) It requ...

A good transition-state analog: A) binds covalently to the enzyme. B) binds to the enzyme more tightly than the substrate. C) binds very weakly to the enzyme. D) is too unstable to isolate. E) must be almost identical to the substrate. - Answer-binds to the enzyme more tightly than the substrate. A major advance in the application of mass spectrometry to macromolecules came with the development of techniques to overcome which of the following problems? A) Macromolecules were insoluble i...

In a bacterial cell, the DNA is in the: A) cell envelope. B) cell membrane. C) nucleoid. D) nucleus. E) ribosomes. Correct Answer-nucleoid A major change occurring in the evolution of eukaryotes from prokaryotes was the development of: A) DNA. B) photosynthetic capability. C) plasma membranes. D) ribosomes. E) the nucleus. Correct Answer-the nucleus. In eukaryotes, the nucleus is enclosed by a double membrane called the: A) cell membrane. B) nuclear envelope. C) nu...

A good transition-state analog: - Answer-binds to the enzyme more tightly than the substrate A major advance in the application of mass spectrometry to macromolecules came with the development of techniques to overcome which of the following problems? - Answer-Mass spectrometric analysis involved molecules in the gas phase A major change occurring in the evolution of eukaryotes from prokaryotes was the development of: - Answer-the nucleus A metabolic pathway proceeds according to the schem...

In a bacterial cell, the DNA is in the: A) cell envelope. B) cell membrane. C) nucleoid. D) nucleus. E) ribosomes. Ans: nucleoid A major change occurring in the evolution of eukaryotes from prokaryotes was the development of: A) DNA. B) photosynthetic capability. C) plasma membranes. D) ribosomes. E) the nucleus. Ans: the nucleus. In eukaryotes, the nucleus is enclosed by a double membrane called the: A) cell membrane. B) nuclear envelope. C) nucleolus. D) nucleopl...

A nonapeptide was determined to have the following amino acid com position: (Lys)2, (Gly) 2, (Phe) 2, His, Leu, Met. The native peptide was incubated with 1-fluoro-2,4- dinitrobenzene (FDNB) and then hydrolyzed; 2,4-dinitrophenylhistidine was identified by HPLC. When the native peptide was exposed to cyanogen bromide(CNBr), an octapeptide and free glycine were recovered. Incubation of the native peptide with trypsin gave a pentapeptide, a tripeptide, and free Lys. 2,4-Dinitrophenyl-histidin...

A dynamic steady state results when A. a molecule stops being degraded. B. an organism is at equilibrium with its surroundings. C. there is no net energy transfer. D. the rate of intake or synthesis of a molecule equals the rate of its disappearance - Choice D is correct. A dynamic steady state results when the rate of synthesis or intake of a molecule equals the rate of its breakdown, consumption, or conversion into some other product. For example, a dynamic steady state in the blood ma...