Bcmb 406b test 1 - Study guides, Class notes & Summaries

what variables are considered in primer design - length, sequence, %GC, Tm, Ta, 3'end stability, 5' end stability, secondary structures why is the formation of secondary structures in primers especially undesirable - reduces primer available to use to make product standard denaturation temp and time (initial and denaturation) - 94C 1-5min initial 20-45 sec std annealing temp and time - temp varies between protocols, 30s std elongation temp and time - temp depends on polymerase u...

what two criteria need to be meet when picking a replacement residue in SDM - Answer remove charge of functional group of original residue be similar or smaller in size What are the most significant problems in primer design - Answer secondary structures (loses primer), 3' end stability (mispriming), Tm difference (hard to set up) Primer length - Answer 18-22bp

what variables are considered in primer design length, sequence, %GC, Tm, Ta, 3'end stability, 5' end stability, secondary structures why is the formation of secondary structures in primers especially undesirable reduces primer available to use to make product Previous Play Next Rewind 10 seconds Move forward 10 seconds Unmute 0:00 / 0:15 Full screen Brainpower Read More standard denaturation temp and time (initial and denaturation) 94C 1-5min initial 20-45 sec...

BCMB 406B Test 1Latest Update ...

BCMB 406B Midterm 1 Test Latest Update ...

BCMB 406B Midterm 1 Lab 1 Test Latest Update ...

Lab 1 - Exercise 1: Primer Analysis and PCR Trouble-Shooting - Answer Compared and contrasted two primer pairs to find the qualities that contribute to the likelihood of a given primer pair producing the desired product. - NetPrimer Lab 1 - Exercise 2: Primer Design from a Target Sequence - Answer 1. Nucleotide BLAST (BlastN): identify the sequence. --> indicate organism of origin, size of assigned DNA sequence, and the name of the protein it encodes.

BCMB 406B - Lab 1 Test With Complete Solution ...