Polymerase cha - Study guides, Class notes & Summaries

Polymerase Chain Reaction (PCR) 1. Polymerase chain reaction (PCR) allows for creation of 2. PCR product can be visualized via

We are going to use the blood sample you collected at the crime scene. What needs to happen before the blood sample can be used for PCR? a) DNA has to be isolated from the cells b) No action is needed c) You should shake the tube d) The blood has to be frozen. - Answer a) DNA has to be isolated from the cells What do you need to do each time before using pipette to collect liquid? a) Put on a used pipette tip b) Polish the pipette c) Shake the pipette d) Put on a new, sterile pipette tip -...

What needs to be done to the sample of blood before PCR? - Answer DNA must be isolated from the cells. How did you collect quids in the lab? - Answer A pipette What do you need to do each time before using a pipette to collect liquid? - Answer Put on a new, sterile pipette tip. What is the function of primers in a PCR reaction? - Answer Bind specific sites on DNA Great that you throw away the used pipette tip, why is it important to change the pipette tip? - Answer to ...

What needs to be done before PCH can be performed - Answer DNA has to be isolated from the Cells How did you collect liquid in a lab - Answer Using a pipette What do you need to do each time before using a pipette to collect liquid - Answer Put on a new sterile pipette tip What is the fiction of primers in a PCR reaction? - Answer Bind to specific sites on the DNA Wh is it important to change the pipette tip? - Answer To avoid cross contamination For which enzyme ar...

The Bradford assay only works at low protein concentrations (from 0.05 to 0.5 mg/mL protein) How can we reduce the concentration of protein in our sample? - Answer dilute the sample what is the volume range of the P1000 - Answer 200 uL - 1000 uL how would you set the volume to pipette 20 uL with the P20? - Answer "200" how much protein do you need to add to the 800 uL buffer in the first microcentrifuge tube to make a 1:5 dilution? - Answer 200 uL how much fluid from th...

Describe the purpose of PCR - Answer Polymerase chain reaction is a technique used to target specific fragments of DNA and artificially amplify (increase their quantity) them. Explain the use of primers in PCR - Answer The primer is an artificial strand of DNA that is made with a complimentary base sequence to the beginning of the DNA fragment to be amplified. It is used to locate the specific DNA target sequence to be copied Describe the process of PCR - Answer 1. The DNA fragmen...

We are going to use the blood sample you collected at the crime scene. What needs to happen before the blood sample can be used for PCR? a) DNA has to be isolated from the cells b) No action is needed c) You should shake the tube d) The blood has to be frozen. a) DNA has to be isolated from the cells What do you need to do each time before using pipette to collect liquid? a) Put on a used pipette tip b) Polish the pipette c) Shake the pipette d) Put on a new, sterile pipette tip d) Put ...

What needs to be done to the sample of blood before PCR? DNA must be isolated from the cells. How did you collect quids in the lab? A pipette What do you need to do each time before using a pipette to collect liquid? Put on a new, sterile pipette tip. What is the function of primers in a PCR reaction? Bind specific sites on DNA Great that you throw away the used pipette tip, why is it important to change the pipette tip? to avoid cross contamination For wh...

What is the function of DNA? DNA stores information Which Cell contains DNA? A white blood cell DNA is located instead the... Nucleus What type of structure does DNA have? double helix What is the complementary DNA sequence for the following sequence: 5'-AATTCGCGG-3' 3'-TTAAGCGCC-5' Which of the following nucleotides is not a part of DNA? Uracil Where is uracil used? In RNA What approach would you take to find out which blood sample cam...