Santa cruz biol 101l - Study guides, Class notes & Summaries

Naomi Schilpp Giulia Gurun BioL 101L 7 May 2019 Insertion of egfp Into pET41a-(+) to Create a Recombinant Plasmid Results: Ligation of egfp insert into pET41A-(+) vector A ligation reaction was used to create recombinant expression plasmid of pET41a- (+)/Enhanced Green Fluorescent Protein (egfp). Five ligations were performed, three of which were controls. The positive control contained only uncut pET41-a(+)/egfp recombinant plasmid DNA and sterile water. Two negative controls were don...