Southern blotting - Study guides, Class notes & Summaries

MCB 2050 Midterm W21 – Dr. Yankulov via Respondus Lockdown Browser and Webcam PART A – Questions from tutorial Question 1. You are studying the genes involved in embryonal development. Which of the following techniques could be used to visualize the locations with high gene expression of a particular gene in a whole mounted embryo? a) Western blotting b) Northern blotting c) Southern blotting d) Immunoprecipitation e) RNA in situ hybridization Question 2. Why is Taq DNA polymerase ...

What modification neutralizes the charges on histones and looses up the interactions between histones and DNA? - ANSWER ACETYLATION Two types of post-transcriptional modifications that take place in the mRNA of eukaryotes - ANSWER the addition of a CAP at the 5' end of the transcript and the addition of a POLY A sequence at the 3' end of the message Nucleic acid blotting is widely used in recombinant DNA technology. In a Southern blot one generally - ANSWER Hybridizes filter-bound DNA wi...

MCB 2050 Molecular Biology of the Cell - W21 Practice Midterm exam questions and answers tips with Dr. Yankulov University of Guelph PART A 1. Which of the following is NOT an essential component of a cloning vector? a. A unique restriction endonuclease cleavage b. A dominant selectable marker gene c. An origin of replication d. A Taq polymerase binding site 2. Methylation of DNA sequence is used how by bacteria? a. Methylation of DNA can inhibit cell division b. Methyla...

What modification neutralizes the charges on histones and looses up the interactions between histones and DNA? - Answer- ACETYLATION Two types of post-transcriptional modifications that take place in the mRNA of eukaryotes - Answer- the addition of a CAP at the 5' end of the transcript and the addition of a POLY A sequence at the 3' end of the message Nucleic acid blotting is widely used in recombinant DNA technology. In a Southern blot one generally - Answer- Hybridizes filter-bound DNA...

Gel Electrophoresis Questions and Answers Rated A+ What role does gel electrophoresis play in gene therapy research? Gel electrophoresis is used to analyze the delivery and integration of therapeutic genes into target cells, helping assess the effectiveness of gene therapy approaches. How can gel electrophoresis help identify pathogens in a clinical setting? By amplifying specific genetic markers of pathogens and separating them using gel electrophoresis, researchers can quickly ide...

A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as A. southern blotting B. northern blotting C. polymerase chain reaction D. DNA replication

They can also be designed to only bind to one version of an allele - allele specific oligonucleotides (ASO). This can be used with PCR to report on whether samples contain certain mutations or polymorphisms. Southern Blotting - Answer Transfer of macromolecules onto a solid-phase membranous support such as nylon or nitrocellulose. This is done after gel electrophoresis and before hybridization in order to visualize results of these tests. Southern blotting refers to DNA blotting

Adaptive immunity - Answer- specific Two types of adaptive immunity - Answer- Humoral immunity- B cells regulating antibodies Cell Mediated Immunity- T cells regulating innate immunity - Answer- non-specific phosphodiester linkage - Answer- covalent bonds that join adjacent nucleotides between the -OH group of the 3' carbon of one nucleotide and the phosphate on the 5' carbon of the next disulfide bonds - Answer- 2 cysteine residues can covalently bond to form disulfide bonds whic...

Craniofacial Biology, Exam 1 with Practice Questions and Answers Name two specific purposes for cloning foreign DNA into plasmid vectors ANSWER protein drug production (like insulin) and gene therapy Briefly describe the cloning process ANSWER -restriction endonuclease digests and inserts the DNA into a vector with ligation - introduce newly ligated DNA into host cell (like E coli) -identify bacteria that have taken up the proper + vector Where to RE come from and what do Restriction ...

BCHM102 Midterm Exam Practice Questions and Answers aminoacyl tRNA synthetase requires ______ energy - Correct Answer️️ -ATP translocation requires ________ energy - Correct Answer️️ -GTP two methods of translation termination - Correct Answer️️ -hairpin formation and rho protein post-transcriptional modification - Correct Answer️️ -the modifications that occur to an mRNA transcript before it is exported from the nucleus as mature mRNA. Include: splicing of introns, addit...