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Question: This is MSA and the side labelled "1" has turned yellow. Which Staph does this indicate? Ans: Staph aureus Question: This is an EMB plate. What does the test result of "1" indicate? Ans: Lactose Negative Question: This is an EMB plate. What does the test result of "2" indicate? Ans: Lactose Positive Question: This is an EMB plate. What is indicated by "3?" Ans: E. Coli Question: This is a starch plate. What does the clear boundary around this colony ...

Question: What is the purpose of the oxidase test? Ans: -The tests is used to identify bacteria containing the respiratory enzyme cytochrome c oxidase as an electron acceptor in the ETC. Question: How is the oxidase test performed? Ans: 1. Transfer bacterium to the paper 2. Add drops of complex A & B to bacterium 3. Observe for color change within 30 seconds Question: Why should you read the results of the oxidase test within 30 seconds? SERIES Ans: -The reagents of this test are un...

Question: hanging drop steps: Ans: 1. Vaseline around cover slip 2. micro organism in center of cover slip 3. place concave slide on cover slip Question: Gram stain steps: Ans: 1. Crystal Violet 2. Gram's Iodine 3. Ethyl Alcohol 4. Safranin Red Question: negative stain steps: SERIES Ans: 1. nigrosin loop ria 4. slide to spread mixture 5. air dry Question: acid-fast stain steps: Ans: 1. carbolfuchsin 2. acid alcohol 3. methylene blue 4. wash & dry Question: simple s...

Question: The ability of a microscope to reveal fine detail is known as? Ans: resolution Question: The 10X eyepiece used in combination with a 45X objective produces a magnification of? Ans: 450X Question: Another name for the 100X objective lens? Ans: oil immersion Question: when putting the microscope away, which objective should be in place? Ans: low power Question: What part of the microscope regulates the amount of light entering? Ans: condenser SERIES Question: what part of...

Question: autoclave Ans: a steam sterilizer used to sterilize lab equipment at 121 Celsius or 249 Fahrenheit Question: inocculation loop Ans: to transfer a culture of microorganisms from point A to point B by streaking the media (first step is to always sterilize the loop) Question: inocculation needle Ans: transfer a culture of microorganisms by stabbing the media. (first step is to always sterilize the needle) Question: weigh balance Ans: measures mass Question: stir plate Ans: ...

Question: autotroph Ans: nutritionally deficient mutants that have lost the ability to synthesize a particular enzyme many microorganisms in this group: - can carry out photosynthesis - usually do not cause disease - synthesize organic compounds to obtain energy Question: heterotroph Ans: an organism that uses compounds to produce biomolecules Question: autoclave Ans: an instrument for sterilization by means of moist heat under pressure Question: Autoclaving combines ____ and ____ to...

Question: Secondary Containment Ans: protection of the outside lab environment from exposure to infectious organisms Question: Disposal of biohazards Ans: Place biohazard material in marked bag or container; must be tagged or color coded Question: Disposal of Glass waste Ans: can be washed and reused after autoclaving (Kills station) Question: Disposal of plastic waste Ans: Melt in autoclave to dispose after decontaminated Question: Office hours and location Ans: Zurn 207 Monday/Wed...

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Question: Heat Fixing Ans: kills organisms, keeps organisms stuck to slide, allows the specimen to be stained more easily Question: Aseptic Technique Ans: preparing and handling sterile products in a manner that prevents microbial contamination Question: Gram Staining Procedure SERIES Ans: 1. Heat fix bacterial smear 2. Cover with Crystal Violet (Primary Stain) and wait 60 seconds 3. Rinse slide with distilled water until stain is removed 4. Cover with Gram's Iodine (Mordant) an...

Question: At what magnification do you use the oil immersion technique? Ans: 100x. Question: Total Magnification = Ans: Magnification (Objective) x Magnification (Ocular) Question: Important Parts of the Microscope Ans: Framework, Stage, and Lens System Question: Framework Ans: Arm and base are the structural parts of the microscope which support the basic frame. Question: Stage Ans: Holds the slide. The mechanical stage clamps the slide and moves the slide around the stage. SERIE...