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MCB 253 Final Exam questions and verified answers
blocking buffers - Used to prevent nonspecific binding interactions between the antibodies and 
nitrocellulose membrane. Added after transfer to nitrocellulose before antibody staining. 
10X TBST: designed for washing the blots in between stains and 5%nonfat dried milk. 
bromophenol blue - ph marker. used to indicated when the protein reached the bottom of the 
electrophoresis 
Coomassie Blue - a dye that stains proteins blue and allows them to be visualized. it binds to arg, lys, 
his residues ...
- Exam (elaborations)
- • 6 pages •
blocking buffers - Used to prevent nonspecific binding interactions between the antibodies and 
nitrocellulose membrane. Added after transfer to nitrocellulose before antibody staining. 
10X TBST: designed for washing the blots in between stains and 5%nonfat dried milk. 
bromophenol blue - ph marker. used to indicated when the protein reached the bottom of the 
electrophoresis 
Coomassie Blue - a dye that stains proteins blue and allows them to be visualized. it binds to arg, lys, 
his residues ...
Bio 253 Exam 3 hondros questions and answers
_____ law states that the volume of a gas is inversely proportional to its pressure. - Boyle's 
A common condition characterized by acute inflammation of the tracheobronchial tree is - acute 
bronchitis. Correct 
A right shift of the oxygen-hemoglobin dissociation curve due to increased partial pressure of carbon 
dioxide is also known as - the Bohr effect. Correct 
According to the law of partial pressures, the partial pressure of gas in a mixture of gases is - directly 
related to the concent...
- Exam (elaborations)
- • 5 pages •
_____ law states that the volume of a gas is inversely proportional to its pressure. - Boyle's 
A common condition characterized by acute inflammation of the tracheobronchial tree is - acute 
bronchitis. Correct 
A right shift of the oxygen-hemoglobin dissociation curve due to increased partial pressure of carbon 
dioxide is also known as - the Bohr effect. Correct 
According to the law of partial pressures, the partial pressure of gas in a mixture of gases is - directly 
related to the concent...
Bio 253 Exam #3 Practice Questions and answers
A rapid repolarization of the membrane caused by K+ leaving the cell occurs in ? - neurons, in 
contractile cardiac cells, and autorhythmic cardiac cells 
asynchronous recruitment of skeletal muscle - slows the onset of fatigue 
blood in the venae cavae flows into the _____. - right atrium 
capillary exchange is facilitated by - large surface area of capillaries 
creatine phosphate is an important source of skeletal muscle ATP because - it is catalyzed in the 
presence of ADP to produce creatine...
- Exam (elaborations)
- • 3 pages •
A rapid repolarization of the membrane caused by K+ leaving the cell occurs in ? - neurons, in 
contractile cardiac cells, and autorhythmic cardiac cells 
asynchronous recruitment of skeletal muscle - slows the onset of fatigue 
blood in the venae cavae flows into the _____. - right atrium 
capillary exchange is facilitated by - large surface area of capillaries 
creatine phosphate is an important source of skeletal muscle ATP because - it is catalyzed in the 
presence of ADP to produce creatine...
BIO 253: Exam 5 Review (Ch. 12-14 Questions with accurate verified answers
1. convey sensory and motor impulses 
2. information integration - spinal cord functions 
100 million - how many neurons does the spinal cord have 
12 - how many cranial nerves are there? 
31 - how many pairs of spinal cord nerves are there? 
85 billion - how many neurons does the brain have 
a fibers - the largest diameter axons (5-20 um) and are myelinated. these fibers have a brief absolute 
refractory period and conduction nerve impulses at a speed of 12-130 m/sec. the axons of sensory 
neur...
- Exam (elaborations)
- • 28 pages •
1. convey sensory and motor impulses 
2. information integration - spinal cord functions 
100 million - how many neurons does the spinal cord have 
12 - how many cranial nerves are there? 
31 - how many pairs of spinal cord nerves are there? 
85 billion - how many neurons does the brain have 
a fibers - the largest diameter axons (5-20 um) and are myelinated. these fibers have a brief absolute 
refractory period and conduction nerve impulses at a speed of 12-130 m/sec. the axons of sensory 
neur...
Bio 253 Questions with Accurate and Verified Aswers
2 agranulocyte (WBC's) - 1. lymphocyte - (think lymph node) antibodies 
2. monocytes - largest, phagocytes 
2 mechanisms for 02 transport - 1. combines with hemoglobin and makes oxyhemoglobin (most 
important) 
2. dissolves 02 in plasma 
3 granulocyte (WBC's) - 1. neutrophil - most active phagocyte 
2. basophils - histamine/ heparin 
3. eosinophil - parasitic worm 
3 mechanisms for C02 transport - 1. 70% carried in plasma as bicarbonate 
2. 20% combined with NH2 (amine) of hemoglobin and other...
- Exam (elaborations)
- • 7 pages •
2 agranulocyte (WBC's) - 1. lymphocyte - (think lymph node) antibodies 
2. monocytes - largest, phagocytes 
2 mechanisms for 02 transport - 1. combines with hemoglobin and makes oxyhemoglobin (most 
important) 
2. dissolves 02 in plasma 
3 granulocyte (WBC's) - 1. neutrophil - most active phagocyte 
2. basophils - histamine/ heparin 
3. eosinophil - parasitic worm 
3 mechanisms for C02 transport - 1. 70% carried in plasma as bicarbonate 
2. 20% combined with NH2 (amine) of hemoglobin and other...
MCB 253 Final Questions and Correct Answers
Anode, cathode - Anode (+) membrane <--- cathode (-) gel. 
B-metacaptoethonol - breaks disulfide linkages 
Blocking buffer. Types? - Used to prevent nonspecific binding interactions between the antibodies 
and nitrocellulose membrane. Added after transfer to nitrocellulose before antibody staining. 
10X TBST: designed for washing the blots in between stains and 5%nonfat dried milk. 
Bromophenol blue - PH marker 
Continous gel - gel and tank buffers are the same, single phase gel. Examples are...
- Exam (elaborations)
- • 5 pages •
Anode, cathode - Anode (+) membrane <--- cathode (-) gel. 
B-metacaptoethonol - breaks disulfide linkages 
Blocking buffer. Types? - Used to prevent nonspecific binding interactions between the antibodies 
and nitrocellulose membrane. Added after transfer to nitrocellulose before antibody staining. 
10X TBST: designed for washing the blots in between stains and 5%nonfat dried milk. 
Bromophenol blue - PH marker 
Continous gel - gel and tank buffers are the same, single phase gel. Examples are...
BIOL 253 lab final UNC questions with accurate answers
(agonist/antagonist) muscles are sometimes called "prime movers" as they are the primary muscle 
responsible for generating movement through their contraction. - agonist 
(agonist/antagonist) muscles control (or oppose) specific movement, and return the muscle to its initial 
position. - antagonist 
A child has recently been diagnosed with Duchenne muscular dystrophy, an X-linked, recessive 
condition. The parents are receiving genetic counseling prior to planning another pregnancy. Which of 
...
- Exam (elaborations)
- • 16 pages •
(agonist/antagonist) muscles are sometimes called "prime movers" as they are the primary muscle 
responsible for generating movement through their contraction. - agonist 
(agonist/antagonist) muscles control (or oppose) specific movement, and return the muscle to its initial 
position. - antagonist 
A child has recently been diagnosed with Duchenne muscular dystrophy, an X-linked, recessive 
condition. The parents are receiving genetic counseling prior to planning another pregnancy. Which of 
...
Hondros Bio 253 questions and answers
Abducens nerve (CN VI) - abduction of eye, proprioception 
Accessory nerve (CN XI) - shoulder movements, turning movements of the head, proprioception 
Acetylcholine - chemical structure, found in junctions with motor effectors (skeletal muscles, glands, 
many parts of the brain 
Afferent neurons - Brings signal to CNS, *sensory 
Alveolar Ventilation - Volume of inspired air that actually reaches the alveoli, helps determine 
amount of oxygen that diffuses into the blood 
Arteries - conduct bloo...
- Exam (elaborations)
- • 7 pages •
Abducens nerve (CN VI) - abduction of eye, proprioception 
Accessory nerve (CN XI) - shoulder movements, turning movements of the head, proprioception 
Acetylcholine - chemical structure, found in junctions with motor effectors (skeletal muscles, glands, 
many parts of the brain 
Afferent neurons - Brings signal to CNS, *sensory 
Alveolar Ventilation - Volume of inspired air that actually reaches the alveoli, helps determine 
amount of oxygen that diffuses into the blood 
Arteries - conduct bloo...
Bio 253 questions and verified answers
actocholine - which hormone causes contraction/constriction? 
ADH - what is produced when dehydrated 
ADH - what is produced when we are dehydrated 
afferent fibers - what carry sensory signals from receptors to the CNS 
amino acids - What are the building blocks of proteins? 
anti-diuretic hormone - What does ADH stand for? 
auricle, external acoustic meatus, tympanic membrane, auditory ossicles, cochlea - what is the 
pathway of transmission from the external ear in? 
away form the CNS - which...
- Exam (elaborations)
- • 5 pages •
actocholine - which hormone causes contraction/constriction? 
ADH - what is produced when dehydrated 
ADH - what is produced when we are dehydrated 
afferent fibers - what carry sensory signals from receptors to the CNS 
amino acids - What are the building blocks of proteins? 
anti-diuretic hormone - What does ADH stand for? 
auricle, external acoustic meatus, tympanic membrane, auditory ossicles, cochlea - what is the 
pathway of transmission from the external ear in? 
away form the CNS - which...
BIOL 253L Final Exam Questions with Answers
?? - Increase during inspiration to supply lungs, decrease during expiration to baseline 
?? - increase in CO2 
?? - increase in smooth muscle tone 
?? - increases HR and CO to supply muscles, increase in RR and TV to oxygenate 
Advantage of turbulent flow: - - allows all of the inspired air to be exposed to the upper airways 
epithelial cells 
- allows more efficient removal of dust and other particles from the inspired air 
Afterload - the active wall tension placed on the heart during systole...
- Exam (elaborations)
- • 13 pages •
?? - Increase during inspiration to supply lungs, decrease during expiration to baseline 
?? - increase in CO2 
?? - increase in smooth muscle tone 
?? - increases HR and CO to supply muscles, increase in RR and TV to oxygenate 
Advantage of turbulent flow: - - allows all of the inspired air to be exposed to the upper airways 
epithelial cells 
- allows more efficient removal of dust and other particles from the inspired air 
Afterload - the active wall tension placed on the heart during systole...