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BACE Exam 2.0 Biological Systems 2023
All life forms are made from one or more cells. 
Cells only arise from pre-existing cells. 
The cell is the smallest form of life. - What are the three components the cell theory? 
 
Prokaryotes are unable to properly fold and assemble more complicated eukaryotic proteins. - What is a disadvantage to using prokaryotes in the production of biopharmaceuticals ? 
 
Plasma membrane - regulates the flow of molecules into and out of the cell 
 
37 C - What is the temperature for the maximum growth rat...
- Exam (elaborations)
- • 4 pages •
All life forms are made from one or more cells. 
Cells only arise from pre-existing cells. 
The cell is the smallest form of life. - What are the three components the cell theory? 
 
Prokaryotes are unable to properly fold and assemble more complicated eukaryotic proteins. - What is a disadvantage to using prokaryotes in the production of biopharmaceuticals ? 
 
Plasma membrane - regulates the flow of molecules into and out of the cell 
 
37 C - What is the temperature for the maximum growth rat...
BACE written/practical exam - another bace test
after successful bacterial transformation with plasmid DNA, you plan to isolate the plasmid DNA from the genomic DNA using a miniprep. what should you not do? - use water to precipitate and wash DNA 
 
how would you describe a tertiary protein structure? - the three dimensional shape of the polypeptide chain once is folded 
 
what is true about about protein synthesis? - transfer RNA (tRNA) molecules that carry amino acids 
 
you observe an unknown cell under a microscope. a nucleus is visible. ...
- Exam (elaborations)
- • 6 pages •
after successful bacterial transformation with plasmid DNA, you plan to isolate the plasmid DNA from the genomic DNA using a miniprep. what should you not do? - use water to precipitate and wash DNA 
 
how would you describe a tertiary protein structure? - the three dimensional shape of the polypeptide chain once is folded 
 
what is true about about protein synthesis? - transfer RNA (tRNA) molecules that carry amino acids 
 
you observe an unknown cell under a microscope. a nucleus is visible. ...
BACE written/practical exam - biotech 20
what is not considered a significant advance in PCR methodology - the use of agarose gel electrophoresis to visualize amplified products 
 
describe DNA ligase - joining pieces of DNA together 
 
describe heat stable DNA polymerase - performing PCR reactions 
 
describe protease - degrading protein 
 
describe restriction enzymes - digesting DNA 
 
what is a negative outcome of growing or consuming genetically modified food crops? - decrease genetic diversity 
 
bioethics is best described as - ...
- Exam (elaborations)
- • 3 pages •
what is not considered a significant advance in PCR methodology - the use of agarose gel electrophoresis to visualize amplified products 
 
describe DNA ligase - joining pieces of DNA together 
 
describe heat stable DNA polymerase - performing PCR reactions 
 
describe protease - degrading protein 
 
describe restriction enzymes - digesting DNA 
 
what is a negative outcome of growing or consuming genetically modified food crops? - decrease genetic diversity 
 
bioethics is best described as - ...
BACE test Question and Answer
what represents the correct order of assembly in the cell - Amino acid, polypeptide, protein 
 
t/f: A single base pair change in a gene's DNA sequence can affect the shape and function of the protein it encodes. - true 
 
t/f:The three-dimensional structure of a protein is largely determined by the amino acid sequence. - true 
 
A structural gene's code is 5' TAC ATG CCC TTA ATC 3', what will the mRNA transcript be? - 3' AUG UAC GGG AAU UAG 5' 
 
Circular, long DNA containing several thou...
- Exam (elaborations)
- • 10 pages •
what represents the correct order of assembly in the cell - Amino acid, polypeptide, protein 
 
t/f: A single base pair change in a gene's DNA sequence can affect the shape and function of the protein it encodes. - true 
 
t/f:The three-dimensional structure of a protein is largely determined by the amino acid sequence. - true 
 
A structural gene's code is 5' TAC ATG CCC TTA ATC 3', what will the mRNA transcript be? - 3' AUG UAC GGG AAU UAG 5' 
 
Circular, long DNA containing several thou...
BACE Practice Exam Questions Version A
NaCl is a: 
-nonpolar covalent 
-ionic 
-polar covalent - ionic 
 
H2O is a: 
-nonpolar covalent 
-ionic 
-polar covalent - polar covalent 
 
CH4 is a: 
-nonpolar covalent 
-ionic 
-polar covalent - nonpolar covalent 
 
What should be used to calculate the amount of compound required to make a solution? - molecular weight 
 
What is an example of a carbohydrate and its associated functions? - Example is cellulose. Functions are energy storage, receptors, structure of plant cell wall 
 
What is ...
- Exam (elaborations)
- • 10 pages •
NaCl is a: 
-nonpolar covalent 
-ionic 
-polar covalent - ionic 
 
H2O is a: 
-nonpolar covalent 
-ionic 
-polar covalent - polar covalent 
 
CH4 is a: 
-nonpolar covalent 
-ionic 
-polar covalent - nonpolar covalent 
 
What should be used to calculate the amount of compound required to make a solution? - molecular weight 
 
What is an example of a carbohydrate and its associated functions? - Example is cellulose. Functions are energy storage, receptors, structure of plant cell wall 
 
What is ...
BACE practical exam
safety shower location - 
 
toxic/poisonous materials - 
 
biohazard - 
 
hot surface - 
 
don't drink the water - 
 
defibrillator or AED location - 
 
eye protection required - 
 
harmful or irritant - 
 
eyewash station - 
 
gloves required - 
 
fire extinguisher location - 
 
first aid station - 
 
protective clothing required - 
 
corrosive - 
 
radioactivity - 
 
oxidizer - 
 
analytical balance - measure mass 
 
autoclave - sterilizes media, trash, and supplies 
 
litmus ...
- Exam (elaborations)
- • 4 pages •
safety shower location - 
 
toxic/poisonous materials - 
 
biohazard - 
 
hot surface - 
 
don't drink the water - 
 
defibrillator or AED location - 
 
eye protection required - 
 
harmful or irritant - 
 
eyewash station - 
 
gloves required - 
 
fire extinguisher location - 
 
first aid station - 
 
protective clothing required - 
 
corrosive - 
 
radioactivity - 
 
oxidizer - 
 
analytical balance - measure mass 
 
autoclave - sterilizes media, trash, and supplies 
 
litmus ...
BACE Practice Exam Version A 3.0 General Topics in Biotechnology
Which of the following is NOT considered a significant advance in PCR methodology? - the use of agarose gel electrophoresis to visualize amplified products 
 
Match the enzyme to its potential use in the laboratory 
 
DNA ligase - joining pieces of DNA together 
 
Match the enzyme to its potential use in the laboratory 
 
Heat-stable DNA polymerase - preforming PCR reactions 
 
Match the enzyme to its potential use in the laboratory 
 
Protease - degrading protein 
 
Match the enzyme to its pote...
- Exam (elaborations)
- • 4 pages •
Which of the following is NOT considered a significant advance in PCR methodology? - the use of agarose gel electrophoresis to visualize amplified products 
 
Match the enzyme to its potential use in the laboratory 
 
DNA ligase - joining pieces of DNA together 
 
Match the enzyme to its potential use in the laboratory 
 
Heat-stable DNA polymerase - preforming PCR reactions 
 
Match the enzyme to its potential use in the laboratory 
 
Protease - degrading protein 
 
Match the enzyme to its pote...
3.0 BACE Exam Question and Answer 2023
The invention of the thermal cycler 
The use of reverse transcriptase from single-stranded RNA 
The use of fluorescently-tagged probes for real-time quantitation of PCR products - SIgnificant Advances in PCR include 
 
the structure of DNA was described, as well as its method of replication - What happened in the 1950s? 
 
the isolation of restriction enzymes - The advent of recombinant DNA technology began with what? 
 
it allowed us to manipulate DNA - What did the advent of recombinant DNA t...
- Exam (elaborations)
- • 7 pages •
The invention of the thermal cycler 
The use of reverse transcriptase from single-stranded RNA 
The use of fluorescently-tagged probes for real-time quantitation of PCR products - SIgnificant Advances in PCR include 
 
the structure of DNA was described, as well as its method of replication - What happened in the 1950s? 
 
the isolation of restriction enzymes - The advent of recombinant DNA technology began with what? 
 
it allowed us to manipulate DNA - What did the advent of recombinant DNA t...
Biotech BACE Exam Question and Answer 2023
The process of making RNA from DNA - Transcription 
 
The process by which RNA is decoded and polypeptides are formed - Translation 
 
Autonomously replicating small circles of DNA present in some bacteria - Plasmids 
 
Modifications of DNA in an organism - Genetic engineering 
 
Parkinson's, diabetes, and cystic fibrosis (CF) are examples of - Genetic disorders 
 
Organism that does not have nucleus or membrane bound organelle, so DNA in contained in cytoplasm - Prokaryotes 
 
Help researchers...
- Exam (elaborations)
- • 5 pages •
The process of making RNA from DNA - Transcription 
 
The process by which RNA is decoded and polypeptides are formed - Translation 
 
Autonomously replicating small circles of DNA present in some bacteria - Plasmids 
 
Modifications of DNA in an organism - Genetic engineering 
 
Parkinson's, diabetes, and cystic fibrosis (CF) are examples of - Genetic disorders 
 
Organism that does not have nucleus or membrane bound organelle, so DNA in contained in cytoplasm - Prokaryotes 
 
Help researchers...
BACE Exam-Knowledge Question and Answer 2023
Clinical Trial- Phase 1 - - Tests drug in healthy individuals 
- Tests for safety, dosage, and side effects 
 
Clinical Trial- Phase 2 - - Tests on larger group of effected individuals 
- Tests for efficacy and side effects 
 
Clinical Trial-Phase 3 - - Tests on new and wider demographic 
- Tests for long term effectiveness and comparisons with other medications 
 
Clinical Trial- FDA Approval - Treatment determined effective and safe for public use 
 
Clinical Trial- Phase 4 - - Continues to te...
- Exam (elaborations)
- • 10 pages •
Clinical Trial- Phase 1 - - Tests drug in healthy individuals 
- Tests for safety, dosage, and side effects 
 
Clinical Trial- Phase 2 - - Tests on larger group of effected individuals 
- Tests for efficacy and side effects 
 
Clinical Trial-Phase 3 - - Tests on new and wider demographic 
- Tests for long term effectiveness and comparisons with other medications 
 
Clinical Trial- FDA Approval - Treatment determined effective and safe for public use 
 
Clinical Trial- Phase 4 - - Continues to te...